Recombinant
RabMAb

Anti-MSH3 antibody [EPR4334(2)] (ab111107)

Overview

  • Product name
    Anti-MSH3 antibody [EPR4334(2)]
    See all MSH3 primary antibodies
  • Description
    Rabbit monoclonal [EPR4334(2)] to MSH3
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human MSH3.

  • Positive control
    • Human colon adenocarcinoma and tonsil tissues; SW480 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab111107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
    • Involvement in disease
      Defects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    • Sequence similarities
      Belongs to the DNA mismatch repair mutS family. MSH3 subfamily.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
    • Alternative names
      • Divergent upstream protein antibody
      • DNA mismatch repair protein antibody
      • DNA mismatch repair protein Msh 3 antibody
      • DNA mismatch repair protein MSH3 antibody
      • DUC 1 antibody
      • DUC1 antibody
      • DUG antibody
      • DUP antibody
      • hMSH3 antibody
      • MGC163306 antibody
      • MGC163308 antibody
      • Mismatch repair protein 1 antibody
      • MRP 1 antibody
      • MRP1 antibody
      • MSH 3 antibody
      • MSH3 antibody
      • MSH3_HUMAN antibody
      • MutS homolog 3 (E. coli) antibody
      • MutS homolog 3 antibody
      see all

    Images

    • ab111107 at 1/500 dilution staining MSH3 in Paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
    • ab111107 at 1/500 dilution staining MSH3 in Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
    • ab111107 at 1/250 dilution staining MSH3 in SW480 cells by Immunofluorescence.

    References

    ab111107 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    I am afraid we do not have small samples of any of our antibodies. Antibody ab111107 may be specific for an epitope that exists only in the native conformation, as the western blot testing is carried out on reduced and denatured samples. We have not te...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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