Overview

  • Product nameAnti-MSH5 antibody
    See all MSH5 primary antibodies
  • Description
    Rabbit polyclonal to MSH5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 144-193 (KQRLLSGNYS FIPDAMTATE KILFLSSIIP FDCLLTPPGD LRFTPIPLLI) of Human MSH5 (NP_079535).

  • Positive control
    • 721_B cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • FunctionInvolved in meiotic recombination. Facilitate crossovers between homologs during meiosis.
  • Tissue specificityUbiquitous, but highly expressed in testis, and thymus.
  • Sequence similaritiesBelongs to the DNA mismatch repair mutS family.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434C1615 antibody
    • G7 antibody
    • hMSH5 antibody
    • MGC2939 antibody
    • MSH 5 antibody
    • MSH5 antibody
    • MSH5_HUMAN antibody
    • mutS (E. coli) homolog 5 antibody
    • mutS homolog 5 antibody
    • mutS homolog 5 (E. coli) antibody
    • MutS protein homolog 5 antibody
    • MutSH5 antibody
    • NG23 antibody
    • OTTHUMP00000029187 antibody
    • OTTHUMP00000029188 antibody
    see all

Anti-MSH5 antibody images

  • Anti-MSH5 antibody (ab83004) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MSH5 antibody (ab83004)

ab83004 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"