Anti-MSI2 antibody (ab114107)
Key features and details
- Rabbit polyclonal to MSI2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MSI2 antibody
See all MSI2 primary antibodies -
Description
Rabbit polyclonal to MSI2 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Guinea pig, Ferret, Lizard, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region between amino acids 250-300 of Human MSI2 (NP_620412.1).
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Positive control
- HeLa whole cell lysate (ab150035).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab114107 was affinity purified using an epitope specific to MSI2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system. -
Tissue specificity
Ubiquitous; detected at low levels. -
Involvement in disease
Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23). -
Sequence similarities
Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cytoplasm. Associated with polysomes. - Information by UniProt
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Database links
- Entrez Gene: 124540 Human
- Entrez Gene: 76626 Mouse
- Omim: 607897 Human
- SwissProt: Q96DH6 Human
- SwissProt: Q920Q6 Mouse
- Unigene: 658922 Human
- Unigene: 400451 Mouse
- Unigene: 428433 Mouse
see all -
Alternative names
- FLJ36569 antibody
- MGC3245 antibody
- Msi2 antibody
see all
Images
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Detection of Human MSI2 by Western blot of Immunoprecipitate.
MSI2 was immunoprecipitated from HeLa whole cell lysate, using ab114107 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). ab114107 was used for the subsequent WB at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab114107 has been referenced in 1 publication.
- Cragle CE et al. Musashi interaction with poly(A)-binding protein is required for activation of target mRNA translation. J Biol Chem 294:10969-10986 (2019). PubMed: 31152063