Customer reviews and FAQs

Overview

  • Product name
    Anti-MSI2 antibody
    See all MSI2 primary antibodies
  • Description
    Rabbit polyclonal to MSI2
  • Tested applications
    Suitable for: ELISA, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Apteronotus leptorhynchus
    Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Caenorhabditis elegans, Chimpanzee, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: LRDYFSKFGE IRECMVMRDP TTKRSRGFGF VTFADPASVD KVLGQPHHEL, corresponding to N terminal amino acids 37-86 of Human MSI2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HepG2 cell lysate, human liver tissue and human heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab50829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • Tissue specificity
    Ubiquitous; detected at low levels.
  • Involvement in disease
    Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
  • Sequence similarities
    Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Cytoplasm. Associated with polysomes.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • FLJ36569 antibody
    • MGC3245 antibody
    • Msi2 antibody
    • MSI2/HOXA9 fusion gene, included antibody
    • MSI2H antibody
    • MSI2H_HUMAN antibody
    • Musashi 2 antibody
    • Musashi homolog 2 antibody
    • Musashi RNA binding protein 2 antibody
    • Musashi, Drosophila, homolog of, 2 antibody
    • Musashi-2 antibody
    • RNA binding protein Musashi homolog 2 antibody
    • RNA-binding protein Musashi homolog 2 antibody
    • WD 40 repeat protein MSI2 antibody
    see all

Anti-MSI2 antibody images

  • Western blot - Anti-MSI2 antibody (ab50829)
    Western blot - Anti-MSI2 antibody (ab50829)
    All lanes : Anti-MSI2 antibody (ab50829) at 2.5

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 35 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody (ab50829)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody (ab50829)
    Ab50829 (8ug/ml) staining human MSI2 in human liver tissue by immunohistochemistry using paraffin embedded tissue. Hepatocytes (indicated with arrow) were positively stained.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody (ab50829)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody (ab50829)
    AB50829 (8ug/ml) staining human MSI2 in human heart tissue by immunohistochemistry using paraffin embedded tissue. Myocardial cells (indicated with arrows) were positively stained.
  • Immunohistochemistry (Frozen sections) - Anti-MSI2 antibody (ab50829)
    Immunohistochemistry (Frozen sections) - Anti-MSI2 antibody (ab50829)Image courtesy of Dr Ruxandra Sirbulescu by Abreview.
    ab50829 staining MSI2 in 16 µm thick transverse sections of adult brown ghost knifefish spinal cord by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using 0.3% Triton X-100, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at room temperature and then incubated with ab50829 at a 1/25 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    See Abreview

  • Western blot - Anti-MSI2 antibody (ab50829)
    Western blot - Anti-MSI2 antibody (ab50829)
    Anti-MSI2 antibody (ab50829) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1:100,000)

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

References for Anti-MSI2 antibody (ab50829)

This product has been referenced in:
  • Sutherland JM  et al. Knockout of RNA Binding Protein MSI2 Impairs Follicle Development in the Mouse Ovary: Characterization of MSI1 and MSI2 during Folliculogenesis. Biomolecules 5:1228-44 (2015). WB ; Mouse . Read more (PubMed: 26131972) »
  • Sutherland JM  et al. Developmental expression of Musashi-1 and Musashi-2 RNA-binding proteins during spermatogenesis: analysis of the deleterious effects of dysregulated expression. Biol Reprod 90:92 (2014). WB, IHC-P ; Mouse . Read more (PubMed: 24671879) »

See all 2 Publications for this product

Publishing research using ab50829? Please let us know so that we can cite the reference in this datasheet.

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2 Abreviews or Q&A

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Votes: Highest First

Western blot abreview for Anti-MSI2 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
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Abcam user community

Verified customer

Submitted Jan 28 2013

Immunohistochemistry (Frozen sections) abreview for Anti-MSI2 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More
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Username

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Dec 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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