- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-MSI2 antibody
See all MSI2 primary antibodies -
DescriptionRabbit polyclonal to MSI2
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, IHC-Fr, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human, Apteronotus leptorhynchus
Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Caenorhabditis elegans, Chimpanzee, Drosophila melanogaster, Zebrafish
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Immunogen
A region within synthetic peptide: LRDYFSKFGE IRECMVMRDP TTKRSRGFGF VTFADPASVD KVLGQPHHEL, corresponding to N terminal amino acids 37-86 of Human MSI2
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Positive control
- HepG2 cell lysate, human liver tissue and human heart tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab50829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 4 - 8 µg/ml. |
Target
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FunctionRNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
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Tissue specificityUbiquitous; detected at low levels.
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Involvement in diseaseNote=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
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Sequence similaritiesBelongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated. -
Cellular localizationCytoplasm. Associated with polysomes.
- Information by UniProt
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Database links
- Entrez Gene: 505542 Cow
- Entrez Gene: 475148 Dog
- Entrez Gene: 124540 Human
- Entrez Gene: 76626 Mouse
- Entrez Gene: 360596 Rat
- Omim: 607897 Human
- SwissProt: Q96DH6 Human
- SwissProt: Q920Q6 Mouse
see all -
Alternative names
- FLJ36569 antibody
- MGC3245 antibody
- Msi2 antibody
see all
Images
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Western blot - Anti-MSI2 antibody (ab50829)All lanes : Anti-MSI2 antibody (ab50829)
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml
Lysates/proteins at 25 µg per lane.
Predicted band size: 35 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody (ab50829)Ab50829 (8ug/ml) staining human MSI2 in human liver tissue by immunohistochemistry using paraffin embedded tissue. Hepatocytes (indicated with arrow) were positively stained. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody (ab50829)AB50829 (8ug/ml) staining human MSI2 in human heart tissue by immunohistochemistry using paraffin embedded tissue. Myocardial cells (indicated with arrows) were positively stained. -
Immunohistochemistry (Frozen sections) - Anti-MSI2 antibody (ab50829)Image courtesy of Dr Ruxandra Sirbulescu by Abreview.ab50829 staining MSI2 in 16 µm thick transverse sections of adult brown ghost knifefish spinal cord by Immunohistochemistry (Frozen sections).
Tissue was fixed in paraformaldehyde, permeabilized using 0.3% Triton X-100, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at room temperature and then incubated with ab50829 at a 1/25 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution. -
Western blot - Anti-MSI2 antibody (ab50829)Anti-MSI2 antibody (ab50829) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG (1:100,000)
Predicted band size: 35 kDa
Observed band size: 35 kDa
References
This product has been referenced in:
- Sutherland JM et al. Knockout of RNA Binding Protein MSI2 Impairs Follicle Development in the Mouse Ovary: Characterization of MSI1 and MSI2 during Folliculogenesis. Biomolecules 5:1228-44 (2015). WB ; Mouse . Read more (PubMed: 26131972) »
- Sutherland JM et al. RNA binding protein Musashi-1 directly targets Msi2 and Erh during early testis germ cell development and interacts with IPO5 upon translocation to the nucleus. FASEB J 29:2759-68 (2015). Read more (PubMed: 25782991) »
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