Overview

  • Product nameAnti-MSI2 antibody
    See all MSI2 primary antibodies
  • Description
    Rabbit polyclonal to MSI2
  • Tested applicationsSuitable for: ELISA, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Apteronotus leptorhynchus
    Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Caenorhabditis elegans, Chimpanzee, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: LRDYFSKFGE IRECMVMRDP TTKRSRGFGF VTFADPASVD KVLGQPHHEL, corresponding to N terminal amino acids 37-86 of Human MSI2

  • Positive control
    • HepG2 cell lysate, human liver tissue and human heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab50829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionRNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • Tissue specificityUbiquitous; detected at low levels.
  • Involvement in diseaseNote=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
  • Sequence similaritiesBelongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationCytoplasm. Associated with polysomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ36569 antibody
    • MGC3245 antibody
    • Msi2 antibody
    • MSI2/HOXA9 fusion gene, included antibody
    • MSI2H antibody
    • MSI2H_HUMAN antibody
    • Musashi 2 antibody
    • Musashi homolog 2 antibody
    • Musashi RNA binding protein 2 antibody
    • Musashi, Drosophila, homolog of, 2 antibody
    • Musashi-2 antibody
    • RNA binding protein Musashi homolog 2 antibody
    • RNA-binding protein Musashi homolog 2 antibody
    • WD 40 repeat protein MSI2 antibody
    see all

Anti-MSI2 antibody images

  • All lanes : Anti-MSI2 antibody (ab50829) at 2.5

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 35 kDa
  • Ab50829 (8ug/ml) staining human MSI2 in human liver tissue by immunohistochemistry using paraffin embedded tissue. Hepatocytes (indicated with arrow) were positively stained.Ab50829 (8ug/ml) staining human MSI2 in human liver tissue by immunohistochemistry using paraffin embedded tissue. Hepatocytes (indicated with arrow) were positively stained.
  • AB50829 (8ug/ml) staining human MSI2 in human heart tissue by immunohistochemistry using paraffin embedded tissue. Myocardial cells (indicated with arrows) were positively stained.AB50829 (8ug/ml) staining human MSI2 in human heart tissue by immunohistochemistry using paraffin embedded tissue. Myocardial cells (indicated with arrows) were positively stained.
  • ab50829 staining MSI2 in 16 µm thick transverse sections of adult brown ghost knifefish spinal cord by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using 0.3% Triton X-100, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at room temperature and then incubated with ab50829 at a 1/25 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    See Abreview

  • Anti-MSI2 antibody (ab50829) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1:100,000)

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

References for Anti-MSI2 antibody (ab50829)

This product has been referenced in:
  • Sutherland JM  et al. Developmental expression of Musashi-1 and Musashi-2 RNA-binding proteins during spermatogenesis: analysis of the deleterious effects of dysregulated expression. Biol Reprod 90:92 (2014). WB, IHC-P ; Mouse . Read more (PubMed: 24671879) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Dec 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"