The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:1562500.
FunctionRNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
Tissue specificityUbiquitous; detected at low levels.
Involvement in diseaseNote=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
Sequence similaritiesBelongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains.
Cellular localizationCytoplasm. Associated with polysomes.
Anti-MSI2 antibody (ab83236) at 1 µg/ml + HepG2 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 35 kDa Observed band size : 35 kDa
References for Anti-MSI2 antibody (ab83236)
This product has been referenced in:
Cox JL et al. The SOX2-interactome in brain cancer cells identifies the requirement of MSI2 and USP9X for the growth of brain tumor cells. PLoS One8:e62857 (2013).
Read more (PubMed: 23667531) »