Anti-MSI2 antibody [EP1305Y] (ab76148)


  • Product name
    Anti-MSI2 antibody [EP1305Y]
    See all MSI2 primary antibodies
  • Description
    Rabbit monoclonal [EP1305Y] to MSI2
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide derived from Human MSI2 (UniProt: Q96DH6).

  • Epitope
    Based on the immunogen sequence for this antibody, it is not predicted to detect the shorter isoforms of MSI2.
  • Positive control
    • Rat brain, Human brain, SW480 and T47D cell lysates and Human placenta tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab76148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).Can be blocked with MSI2 peptide (ab136164).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
    • Tissue specificity
      Ubiquitous; detected at low levels.
    • Involvement in disease
      Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
    • Sequence similarities
      Belongs to the Musashi family.
      Contains 2 RRM (RNA recognition motif) domains.
    • Post-translational
    • Cellular localization
      Cytoplasm. Associated with polysomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ36569 antibody
      • MGC3245 antibody
      • Msi2 antibody
      • MSI2/HOXA9 fusion gene, included antibody
      • MSI2H antibody
      • MSI2H_HUMAN antibody
      • Musashi 2 antibody
      • Musashi homolog 2 antibody
      • Musashi RNA binding protein 2 antibody
      • Musashi, Drosophila, homolog of, 2 antibody
      • Musashi-2 antibody
      • RNA binding protein Musashi homolog 2 antibody
      • RNA-binding protein Musashi homolog 2 antibody
      • WD 40 repeat protein MSI2 antibody
      see all


    • All lanes : Anti-MSI2 antibody [EP1305Y] (ab76148) at 1/2000 dilution

      Lane 1 : Rat brain cell lysates
      Lane 2 : Human brain cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : T47D cell lysates

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Immunohistochemical analysis of paraffin-embedded human placenta with ab76148 at 1/100-1/250 dilution.
    • ICC/IF image of ab76148 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76148, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Overlay histogram showing HeLa cells stained with ab76148 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76148, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.


    This product has been referenced in:
    • Sheng W  et al. Musashi2 promotes the development and progression of pancreatic cancer by down-regulating Numb protein. Oncotarget 8:14359-14373 (2017). WB ; Human . Read more (PubMed: 27092875) »
    • Dong P  et al. Musashi-2, a novel oncoprotein promoting cervical cancer cell growth and invasion, is negatively regulated by p53-induced miR-143 and miR-107 activation. J Exp Clin Cancer Res 36:150 (2017). Read more (PubMed: 29073938) »

    See all 9 Publications for this product

    Customer reviews and Q&As

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (Pancreatic cancer tissues)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer/trypsin
    Pancreatic cancer tissues
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Aug 30 2017

    Thank you for contacting us and for your interest in our products.
    The blocking peptide for the anti-MSI2 antibody ab76148 is now available from our catalogue. The reference is ab136164 and the link to the datasheet is

    Read More

    Thank you for contacting us.

    The immunogen information for this antibody is considered proprietary. However, based on the sequence information, this antibody will not recognize the shorter isoforms of MSI2. I hope this helps, please let me kn...

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