• Product nameAnti-MSI2 antibody [EP1305Y]
    See all MSI2 primary antibodies
  • Description
    Rabbit monoclonal [EP1305Y] to MSI2
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide derived from Human MSI2 (UniProt: Q96DH6).

  • EpitopeBased on the immunogen sequence for this antibody, it is not predicted to detect the shorter isoforms of MSI2.
  • Positive control
    • Rat brain, Human brain, SW480 and T47D cell lysates and Human placenta tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


    Alternative versions available:

    Anti-MSI2 antibody (Alexa Fluor® 488)  [EP1305Y] (ab199067)

    Anti-MSI2 antibody (Alexa Fluor® 647) [EP1305Y] (ab199251)

    Anti-MSI2 antibody (HRP) [EP1305Y] (ab199252)



Our Abpromise guarantee covers the use of ab76148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).Can be blocked with MSI2 peptide (ab136164).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionRNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
    • Tissue specificityUbiquitous; detected at low levels.
    • Involvement in diseaseNote=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
    • Sequence similaritiesBelongs to the Musashi family.
      Contains 2 RRM (RNA recognition motif) domains.
    • Post-translational
    • Cellular localizationCytoplasm. Associated with polysomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ36569 antibody
      • MGC3245 antibody
      • Msi2 antibody
      • MSI2/HOXA9 fusion gene, included antibody
      • MSI2H antibody
      • MSI2H_HUMAN antibody
      • Musashi 2 antibody
      • Musashi homolog 2 antibody
      • Musashi RNA binding protein 2 antibody
      • Musashi, Drosophila, homolog of, 2 antibody
      • Musashi-2 antibody
      • RNA binding protein Musashi homolog 2 antibody
      • RNA-binding protein Musashi homolog 2 antibody
      • WD 40 repeat protein MSI2 antibody
      see all

    Anti-MSI2 antibody [EP1305Y] images

    • All lanes : Anti-MSI2 antibody [EP1305Y] (ab76148) at 1/2000 dilution

      Lane 1 : Rat brain cell lysates
      Lane 2 : Human brain cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : T47D cell lysates

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
      Observed band size : 35 kDa
    • Immunohistochemical analysis of paraffin-embedded human placenta with ab76148 at 1/100-1/250 dilution.
    • ICC/IF image of ab76148 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76148, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Overlay histogram showing HeLa cells stained with ab76148 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76148, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

    References for Anti-MSI2 antibody [EP1305Y] (ab76148)

    This product has been referenced in:
    • Kudinov AE  et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016). WB . Read more (PubMed: 27274057) »
    • Sutherland JM  et al. Knockout of RNA Binding Protein MSI2 Impairs Follicle Development in the Mouse Ovary: Characterization of MSI1 and MSI2 during Folliculogenesis. Biomolecules 5:1228-44 (2015). WB ; Mouse . Read more (PubMed: 26131972) »

    See all 5 Publications for this product

    Product Wall

    Thank you for contacting us and for your interest in our products.
    The blocking peptide for the anti-MSI2 antibody ab76148 is now available from our catalogue. The reference is ab136164 and the link to the datasheet is www.abcam.com/ab136164.

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    Thank you for contacting us.

    The immunogen information for this antibody is considered proprietary. However, based on the sequence information, this antibody will not recognize the shorter isoforms of MSI2. I hope this helps, please let me kn...

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