• Product nameAnti-MSI2 antibody [EP1305Y]
    See all MSI2 primary antibodies
  • Description
    Rabbit monoclonal [EP1305Y] to MSI2
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide derived from Human MSI2 (UniProt: Q96DH6).

  • EpitopeBased on the immunogen sequence for this antibody, it is not predicted to detect the shorter isoforms of MSI2.
  • Positive control
    • Rat brain, Human brain, SW480 and T47D cell lysates and Human placenta tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


    Alternative versions available:

    Anti-MSI2 antibody (Alexa Fluor® 488)  [EP1305Y] (ab199067)

    Anti-MSI2 antibody (Alexa Fluor® 647) [EP1305Y] (ab199251)

    Anti-MSI2 antibody (HRP) [EP1305Y] (ab199252)



Our Abpromise guarantee covers the use of ab76148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).Can be blocked with MSI2 peptide (ab136164).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/30. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionRNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
    • Tissue specificityUbiquitous; detected at low levels.
    • Involvement in diseaseNote=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
    • Sequence similaritiesBelongs to the Musashi family.
      Contains 2 RRM (RNA recognition motif) domains.
    • Post-translational
    • Cellular localizationCytoplasm. Associated with polysomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ36569 antibody
      • MGC3245 antibody
      • Msi2 antibody
      • MSI2/HOXA9 fusion gene, included antibody
      • MSI2H antibody
      • MSI2H_HUMAN antibody
      • Musashi 2 antibody
      • Musashi homolog 2 antibody
      • Musashi RNA binding protein 2 antibody
      • Musashi, Drosophila, homolog of, 2 antibody
      • Musashi-2 antibody
      • RNA binding protein Musashi homolog 2 antibody
      • RNA-binding protein Musashi homolog 2 antibody
      • WD 40 repeat protein MSI2 antibody
      see all

    Anti-MSI2 antibody [EP1305Y] images

    • All lanes : Anti-MSI2 antibody [EP1305Y] (ab76148) at 1/2000 dilution

      Lane 1 : Rat brain cell lysates
      Lane 2 : Human brain cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : T47D cell lysates

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
      Observed band size : 35 kDa
    • Immunohistochemical analysis of paraffin-embedded human placenta with ab76148 at 1/100-1/250 dilution.
    • ICC/IF image of ab76148 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76148, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Overlay histogram showing HeLa cells stained with ab76148 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76148, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

    References for Anti-MSI2 antibody [EP1305Y] (ab76148)

    This product has been referenced in:
    • Wuebben EL  et al. Musashi2 is required for the self-renewal and pluripotency of embryonic stem cells. PLoS One 7:e34827 (2012). WB . Read more (PubMed: 22496868) »
    • Lemieux ME  et al. Inactivation of a Single Copy of Crebbp Selectively Alters Pre-mRNA Processing in Mouse Hematopoietic Stem Cells. PLoS One 6:e24153 (2011). WB ; Mouse . Read more (PubMed: 21901164) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us and for your interest in our products.
    The blocking peptide for the anti-MSI2 antibody ab76148 is now available from our catalogue. The reference is ab136164 and the link to the datasheet is www.abcam.com/ab136164.

    Read More

    Thank you for contacting us.

    The immunogen information for this antibody is considered proprietary. However, based on the sequence information, this antibody will not recognize the shorter isoforms of MSI2. I hope this helps, please let me kn...

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