Anti-MSI2 antibody [OTI2F10] (ab156770)
Key features and details
- Mouse monoclonal [OTI2F10] to MSI2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-MSI2 antibody [OTI2F10]
See all MSI2 primary antibodies -
Description
Mouse monoclonal [OTI2F10] to MSI2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human MSI2. Produced in HEK-293T cells. NP_620412
Database link: Q96DH6 -
Positive control
- WB: HEK-293T transfected with pCMV6-ENTRY MSI2 cDNA; HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC12 and MCF7 cell lysates. IHC-P: Human ovary adenocarcinoma, pancreas, pancreas carcinoma, endometrium adenocarcinoma and prostate tissues. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY MSI2; HeLa cells.
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General notes
The clone number has been updated from 2F10 to OTI2F10, both clone numbers name the same clone.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI2F10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/4000. Predicted molecular weight: 35 kDa.
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IHC-P |
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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Notes |
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WB
1/4000. Predicted molecular weight: 35 kDa. |
IHC-P
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Target
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Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system. -
Tissue specificity
Ubiquitous; detected at low levels. -
Involvement in disease
Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23). -
Sequence similarities
Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cytoplasm. Associated with polysomes. - Information by UniProt
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Database links
- Entrez Gene: 475148 Dog
- Entrez Gene: 124540 Human
- Entrez Gene: 76626 Mouse
- Entrez Gene: 360596 Rat
- Omim: 607897 Human
- SwissProt: Q96DH6 Human
- SwissProt: Q920Q6 Mouse
- Unigene: 658922 Human
see all -
Alternative names
- FLJ36569 antibody
- MGC3245 antibody
- Msi2 antibody
see all
Images
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All lanes : Anti-MSI2 antibody [OTI2F10] (ab156770) at 1/200 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : SVT2 (Mouse cell line) cell lysate
Lane 4 : A549 (Human lung carcinoma cell line) cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 7 : MDCK (Canine kidney cell line) cell lysate
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDa -
Immunofluorescent analysis of COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY MSI2, labeling MSI2 with ab156770 at 1/100 dilution.
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Paraffin-embedded human ovary adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
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Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MSI2 with ab156770 at 1/100 dilution.
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Paraffin-embedded human pancreas tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
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Paraffin-embedded human pancreas adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
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Paraffin-embedded human endometrium adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
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Paraffin-embedded human prostate tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
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All lanes : Anti-MSI2 antibody [OTI2F10] (ab156770) at 1/4000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY MSI2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 35 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab156770 has been referenced in 2 publications.
- Troiano G et al. Immunohistochemical Analysis Revealed a Correlation between Musashi-2 and Cyclin-D1 Expression in Patients with Oral Squamous Cells Carcinoma. Int J Mol Sci 21:N/A (2019). PubMed: 31878037
- Choi YM et al. DBC2/RhoBTB2 functions as a tumor suppressor protein via Musashi-2 ubiquitination in breast cancer. Oncogene 36:2802-2812 (2017). PubMed: 27941885