The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 171 kDa (predicted molecular weight: 145 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3A to DNA. Acts as a corepressor for REST.
SIN3 homolog A transcription regulator (yeast) antibody
SIN3 homolog A transcription regulator antibody
SIN3 transcription regulator homolog A antibody
SIN3A protein antibody
Transcriptional co repressor Sin 3A antibody
Transcriptional co repressor Sin3A antibody
Transcriptional corepressor Sin3a antibody
Transcriptional regulator SIN3A antibody
Anti-mSin3A antibody images
Western blot - mSin3A antibody (ab70727)
All lanes : Anti-mSin3A antibody (ab70727) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg Lane 5 : Whole cell lysate from mouse NIH3T3 cells at 50 µg
Detection of Human mSin3A by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70727 at 3 µg/mg lysate. Subsequent Western blot detection of mSin3A was performed using ab70727 at 1 µg/ml. Detected by chemiluminescence with an exposure time of 10 seconds.
References for Anti-mSin3A antibody (ab70727)
has not yet been referenced specifically in any publications.
Publishing research using ab70727? Please let us know so that we can cite the reference in this datasheet.
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