• Product nameAnti-mSin3A antibody
    See all mSin3A primary antibodies
  • Description
    Rabbit polyclonal to mSin3A
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding a region between residue 375 and 425 of human mSin3A (NP_056292.1).

  • Positive control
    • Whole cell lysates from HeLa, 293T, and mouse NIH3T3 cells.



Our Abpromise guarantee covers the use of ab70727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 171 kDa (predicted molecular weight: 145 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3A to DNA. Acts as a corepressor for REST.
    • Sequence similaritiesContains 3 PAH (paired amphipathic helix) domains.
    • Post-translational
      SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.
    • Cellular localizationNucleus. Nucleus > nucleolus. Recruited to the nucleolus by SAP30L.
    • Information by UniProt
    • Database links
    • Alternative names
      • AW553200 antibody
      • DKFZP434K2235 antibody
      • FLJ90319 antibody
      • Histone deacetylase complex subunit Sin 3a antibody
      • Histone deacetylase complex subunit Sin3a antibody
      • KIAA0700 antibody
      • Kiaa4126 antibody
      • mKIAA4126 antibody
      • Paired amphipathic helix protein Sin 3a antibody
      • Paired amphipathic helix protein Sin3a antibody
      • Sin 3a antibody
      • SIN3 homolog A antibody
      • SIN3 homolog A transcription regulator (yeast) antibody
      • SIN3 homolog A transcription regulator antibody
      • SIN3 transcription regulator homolog A antibody
      • Sin3a antibody
      • SIN3A protein antibody
      • SIN3A_HUMAN antibody
      • Transcriptional co repressor Sin 3A antibody
      • Transcriptional co repressor Sin3A antibody
      • Transcriptional corepressor Sin3a antibody
      • Transcriptional regulator SIN3A antibody
      see all

    Anti-mSin3A antibody images

    • All lanes : Anti-mSin3A antibody (ab70727) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg
      Lane 5 : Whole cell lysate from mouse NIH3T3 cells at 50 µg

      developed using the ECL technique

      Predicted band size : 145 kDa
      Observed band size : 145,171 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds
    • Detection of Human mSin3A by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70727 at 3 µg/mg lysate. Subsequent Western blot detection of mSin3A was performed using ab70727 at 1 µg/ml. Detected by chemiluminescence with an exposure time of 10 seconds.

    References for Anti-mSin3A antibody (ab70727)

    ab70727 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - nuclear (MDA-MB-435)
    Loading amount 50 µg
    Specification MDA-MB-435
    Gel Running Conditions Reduced Denaturing (4-12% BIS-TRIS)
    Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    Submitted May 18 2012