• Product nameAnti-MSK1 antibody
    See all MSK1 primary antibodies
  • Description
    Rabbit polyclonal to MSK1
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 752 and 802 of Human MSK1 (NP_004746.2).

  • Positive control
    • HeLa or 293T cell lysate


Associated products


Our Abpromise guarantee covers the use of ab99412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 90 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionSerine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
  • Tissue specificityWidely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Post-translational
    Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
  • Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 5 antibody
    • EC antibody
    • KS6A5_HUMAN antibody
    • MGC1911 antibody
    • Mitogen and stress activated protein kinase 1 antibody
    • MSPK1 antibody
    • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
    • Nuclear mitogen- and stress-activated protein kinase 1 antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
    • Ribosomal protein S6 kinase 90kDa antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
    • Ribosomal Protein S6 Kinase Alpha 5 antibody
    • Ribosomal protein S6 kinase alpha-5 antibody
    • RLPK antibody
    • RPS6KA5 antibody
    • RSK Like Protein Kinase antibody
    • RSK-like protein kinase antibody
    • RSKL antibody
    • S6K alpha 5 antibody
    • S6K-alpha-5 antibody
    see all

Anti-MSK1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MSK1 with ab99421 at 1/200 (1µg/mg). Detection: DAB.
  • All lanes : Anti-MSK1 antibody (ab99412) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 90 kDa

    Exposure time : 3 minutes
  • Detection of MSK1 in immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded on gel) using ab99412 at 3 µg/mg lysate for IP, and at 1 µg/ml for the subsequent western blot.

    Detection: chemiluminescence with exposure time of 10 seconds.

References for Anti-MSK1 antibody (ab99412)

ab99412 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for confirmation.
One vial of ab99412 has been added to the order 1226955.
Please note that the new lot will be available on 7th January for shipment so there might be a slight delay.
Many thanks for your understanding....

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monit...

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