Anti-MSK1 (phospho S212) antibody (ab79499)
Key features and details
- Rabbit polyclonal to MSK1 (phospho S212)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MSK1 (phospho S212) antibody
See all MSK1 primary antibodies -
Description
Rabbit polyclonal to MSK1 (phospho S212) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human MSK1 (phospho S212).
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Positive control
- IHC: human breast carcinoma tissue WB: extracts from 3T3 cells (treated with EGF at 200 ng/ml for 5mins)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 90 kDa.
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 90 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). -
Tissue specificity
Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsSer-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. -
Cellular localization
Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 9252 Human
- Entrez Gene: 73086 Mouse
- Omim: 603607 Human
- SwissProt: O75582 Human
- SwissProt: Q8C050 Mouse
- Unigene: 510225 Human
- Unigene: 220417 Mouse
- Unigene: 392855 Mouse
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Alternative names
- 90 kDa ribosomal protein S6 kinase 5 antibody
- EC 2.7.11.1 antibody
- KS6A5_HUMAN antibody
see all
Images
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All lanes : Anti-MSK1 (phospho S212) antibody (ab79499) at 1/1000 dilution
Lane 1 : extracts from 3T3 cells (treated
with EGF at 200ng/ml for 5 mins)
Lane 2 : extracts from 3T3 cells (treated
with EGF at 200ng/ml for 5 mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 90 kDa
Observed band size: 90 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, stained with ab79499, at 1/100 dilution, in the absence and presence of the immunising peptide
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab79499 has been referenced in 1 publication.
- Li J et al. Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase. Nat Commun 12:896 (2021). PubMed: 33563994