Overview

  • Product name
    Anti-MSK1 (phospho S212) antibody
    See all MSK1 primary antibodies
  • Description
    Rabbit polyclonal to MSK1 (phospho S212)
  • Host species
    Rabbit
  • Specificity
    ab79499 detects endogenous levels of MSK1 only when phosphorylated at serine 212
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of serine 212 (AYSPFC)

  • Positive control
    • IHC: human breast carcinoma tissue WB: extracts from 3T3 cells (treated with EGF at 200 ng/ml for 5mins)

Properties

Applications

Our Abpromise guarantee covers the use of ab79499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 90 kDa.
IHC-P 1/50 - 1/100.
ELISA 1/40000.

Target

  • Function
    Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
  • Tissue specificity
    Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications
    Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 5 antibody
    • EC 2.7.11.1 antibody
    • KS6A5_HUMAN antibody
    • MGC1911 antibody
    • Mitogen and stress activated protein kinase 1 antibody
    • MSPK1 antibody
    • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
    • Nuclear mitogen- and stress-activated protein kinase 1 antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
    • Ribosomal protein S6 kinase 90kDa antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
    • Ribosomal Protein S6 Kinase Alpha 5 antibody
    • Ribosomal protein S6 kinase alpha-5 antibody
    • RLPK antibody
    • RPS6KA5 antibody
    • RSK Like Protein Kinase antibody
    • RSK-like protein kinase antibody
    • RSKL antibody
    • S6K alpha 5 antibody
    • S6K-alpha-5 antibody
    see all

Images

  • All lanes : Anti-MSK1 (phospho S212) antibody (ab79499) at 1/1000 dilution

    Lane 1 : extracts from 3T3 cells (treated
    with EGF at 200ng/ml for 5 mins)
    Lane 2 : extracts from 3T3 cells (treated
    with EGF at 200ng/ml for 5 mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 90 kDa
    Observed band size: 90 kDa
    Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, stained with ab79499, at 1/100 dilution, in the absence and presence of the immunising peptide

References

ab79499 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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