Overview

  • Product nameAnti-MSK1 (phospho S360) antibody [EP1888Y]
    See all MSK1 primary antibodies
  • Description
    Rabbit monoclonal [EP1888Y] to MSK1 (phospho S360)
  • Specificityab81294 will detect MSK1 phosphorylated on Serine 360.
  • Tested applicationsSuitable for: IP, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phospho peptide corresponding to residues surrounding Serine 360 of human MSK1

  • Positive control
    • HEK293 cell lyasate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab81294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/5000 - 1/20000. Predicted molecular weight: 90 kDa.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionSerine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
    • Tissue specificityWidely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications
      Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
    • Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
    • Information by UniProt
    • Database links
    • Alternative names
      • 90 kDa ribosomal protein S6 kinase 5 antibody
      • EC 2.7.11.1 antibody
      • KS6A5_HUMAN antibody
      • MGC1911 antibody
      • Mitogen and stress activated protein kinase 1 antibody
      • MSPK1 antibody
      • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
      • Nuclear mitogen- and stress-activated protein kinase 1 antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
      • Ribosomal protein S6 kinase 90kDa antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
      • Ribosomal Protein S6 Kinase Alpha 5 antibody
      • Ribosomal protein S6 kinase alpha-5 antibody
      • RLPK antibody
      • RPS6KA5 antibody
      • RSK Like Protein Kinase antibody
      • RSK-like protein kinase antibody
      • RSKL antibody
      • S6K alpha 5 antibody
      • S6K-alpha-5 antibody
      see all

    Anti-MSK1 (phospho S360) antibody [EP1888Y] images

    • All lanes : Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294) at 1/20000 dilution

      Lane 1 : HEK293 cell lyasate, untreated,
      Lane 2 : HEK293 cell lyasate treated with AP.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit. at 1/2000 dilution

      Predicted band size : 90 kDa
      Observed band size : 90 kDa
    • ICC/IF image of ab81294 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81294, neat) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)

    This product has been referenced in:
    • Ufelmann H & Schrenk D Nodularin-triggered apoptosis and hyperphosphorylation of signaling proteins in cultured rat hepatocytes. Toxicol In Vitro 29:16-26 (2015). Read more (PubMed: 25192816) »
    • Fieblinger T  et al. Mechanisms of dopamine D1 receptor-mediated ERK1/2 activation in the parkinsonian striatum and their modulation by metabotropic glutamate receptor type 5. J Neurosci 34:4728-40 (2014). Read more (PubMed: 24672017) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"