Anti-MSK1 (phospho S376) antibody (ab51041)

Overview

  • Product nameAnti-MSK1 (phospho S376) antibody
    See all MSK1 primary antibodies
  • Description
    Rabbit polyclonal to MSK1 (phospho S376)
  • SpecificityThe antibody detects endogenous levels of MSK1 only when phosphorylated at serine 376
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human MSK1 aa 343-392.

  • Positive control
    • HeLa cells treated with PMA

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab51041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 90 kDa).
ELISA 1/20000.

Target

  • FunctionSerine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
  • Tissue specificityWidely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications
    Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
  • Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 5 antibody
    • EC 2.7.11.1 antibody
    • KS6A5_HUMAN antibody
    • MGC1911 antibody
    • Mitogen and stress activated protein kinase 1 antibody
    • MSPK1 antibody
    • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
    • Nuclear mitogen- and stress-activated protein kinase 1 antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
    • Ribosomal protein S6 kinase 90kDa antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
    • Ribosomal Protein S6 Kinase Alpha 5 antibody
    • Ribosomal protein S6 kinase alpha-5 antibody
    • RLPK antibody
    • RPS6KA5 antibody
    • RSK Like Protein Kinase antibody
    • RSK-like protein kinase antibody
    • RSKL antibody
    • S6K alpha 5 antibody
    • S6K-alpha-5 antibody
    see all

Anti-MSK1 (phospho S376) antibody images

  • All lanes : Anti-MSK1 (phospho S376) antibody (ab51041) at 1/500 dilution

    Lane 1 : Hela whole cell lysate, cells treated with PMA (125ng/ml for 15minutes)
    Lane 2 : Hela whole cell lysate


    Predicted band size : 90 kDa

References for Anti-MSK1 (phospho S376) antibody (ab51041)

ab51041 has not yet been referenced specifically in any publications.

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Indeed, I can confirm that we received and published the abreview, thus the discount code x is now active and can be used to geta free primary antibody.

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (pituitary)
Loading amount 10 µg
Specification pituitary
Treatment GnRH 20 min to 120 min
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Mar 30 2012

Thank you very much for contacting us. Of course, she could test the antibody in ChIP instead (or additionally, if she likes). As ChIP has not been tested until now though, we would recommend, testing it with human samples first, as we can guarantee...

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DISCOUNT CODE: xxx Expiration date: dd mm 2012 I am very pleased to hear she would like to accept our offer and test ab51041 in mouse. This code will give 1 free primary antibody before the expiration date. To redeem this offer, please submit an Ab...

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