Anti-MSK1 (phospho T581) antibody (ab59336)

Overview

  • Product nameAnti-MSK1 (phospho T581) antibody
    See all MSK1 primary antibodies
  • Description
    Rabbit polyclonal to MSK1 (phospho T581)
  • Specificityab59336 detects endogenous levels of MSK1 only when phosphorylated at threonine 581. Human: Thr581; Mouse: Thr645.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized phosphopeptide derived from human MSK1 around the phosphorylation site of threonine 581 (LKTPPC).

  • Positive control
    • RAW264.7 nuclear or cytoplasmic lysate
  • General notesStore at -20°C for year

Properties

Applications

Our Abpromise guarantee covers the use of ab59336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa.
ELISA 1/40000.

Target

  • FunctionSerine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
  • Tissue specificityWidely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications
    Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
  • Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 5 antibody
    • EC 2.7.11.1 antibody
    • KS6A5_HUMAN antibody
    • MGC1911 antibody
    • Mitogen and stress activated protein kinase 1 antibody
    • MSPK1 antibody
    • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
    • Nuclear mitogen- and stress-activated protein kinase 1 antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
    • Ribosomal protein S6 kinase 90kDa antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
    • Ribosomal Protein S6 Kinase Alpha 5 antibody
    • Ribosomal protein S6 kinase alpha-5 antibody
    • RLPK antibody
    • RPS6KA5 antibody
    • RSK Like Protein Kinase antibody
    • RSK-like protein kinase antibody
    • RSKL antibody
    • S6K alpha 5 antibody
    • S6K-alpha-5 antibody
    see all

Anti-MSK1 (phospho T581) antibody images

  • All lanes : Anti-MSK1 (phospho T581) antibody (ab59336) at 1/500 dilution

    Lane 1 : Extracts from RAW264.7 cells, treated with UV (5 mins) with no immunizing peptide
    Lane 2 : Extracts from RAW264.7 cells, treated with UV (5 mins) with immunizing peptide


    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-MSK1 (phospho T581) antibody (ab59336)

ab59336 has not yet been referenced specifically in any publications.

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