Overview

  • Product nameAnti-MSK2 / RSK-B antibodySee all MSK2 / RSK-B primary antibodies ...
  • Description
    Rabbit polyclonal to MSK2 / RSK-B
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human MSK2/ RSK-B.

    (Peptide available as ab42280.)

  • Positive control
    • This antibody gave a positive signal in the following cell human lysates: HeLa Nuclear Lysate and HepG2 Whole Cell Lysate.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab42101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).Can be blocked with Human MSK2 / RSK-B peptide (ab42280).
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto-oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 4 antibody
    • EC 2.7.11.1 antibody
    • KS6A4_HUMAN antibody
    • Mitogen and stress activated protein kinase 2 antibody
    • Nuclear mitogen and stress activated protein kinase 2 antibody
    • Nuclear mitogen- and stress-activated protein kinase 2 antibody
    • Ribosomal protein kinase B antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 4 antibody
    • ribosomal protein S6 kinase 90kDa polypeptide 4 antibody
    • Ribosomal protein S6 kinase alpha 4 antibody
    • Ribosomal protein S6 kinase alpha-4 antibody
    • RPS6KA4 antibody
    • RSKB antibody
    • S6K-alpha-4 antibody
    see all

Anti-MSK2 / RSK-B antibody images

  • All lanes : Anti-MSK2 / RSK-B antibody (ab42101) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 150 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab42101 stained MCF7 cells. The cells were 4%PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42101, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2 cells fixed in 4%PFA at 1ug/ml and HeLa, Hek293, HepG2 and MCF7 cells fixed in 100% methanol at 5ug/ml.

References for Anti-MSK2 / RSK-B antibody (ab42101)

This product has been referenced in:
  • Kang JI & Ahn BY HCV-induced PKR activation is stimulated by the mitogen- and stress-activated protein kinase MSK2. Biochem Biophys Res Commun 407:248-53 (2011). WB ; Human . Read more (PubMed: 21385567) »
  • Raymond B  et al. Anthrax lethal toxin impairs IL-8 expression in epithelial cells through inhibition of histone H3 modification. PLoS Pathog 5:e1000359 (2009). WB ; Human . Read more (PubMed: 19343203) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (Rat Schwann cell)
Loading amount 1.2e+006 cells
Specification Rat Schwann cell
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 11 2011

DISCOUNT CODE: ******* Expiration date: ******* I am very pleased to hear you would like to accept our offer and test ab42101 inWB on rat tissue. This code will give you 1 free [primary antibody before the expiration date. To redeem this offer, please ...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (Huh-7)
Loading amount 30 µg
Specification Huh-7
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. JU-IL KANG

Verified customer

Submitted May 19 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"