Anti-MSK2 / RSK-B antibody [2934C1a] (ab71964)

Overview

  • Product name
    Anti-MSK2 / RSK-B antibody [2934C1a]
    See all MSK2 / RSK-B primary antibodies
  • Description
    Mouse monoclonal [2934C1a] to MSK2 / RSK-B
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Monkey
  • Immunogen

    Recombinant fragment: IIDFGFARLR PQSPGVPMQT PCFTLQYAAP ELLAQQGYDE SCDLWSLGVI LYMMLSGQVP FQGASGQGGQ SQAAEIMCKI REGRFSLDGE AWQGVSEEAK ELVRGLLTVD PAKRLKLEGL RGSSWLQDGS ARSSPPLRTP DVLESSGPAV RSGLNATFMA FNRGKREGFF LKSVEN (Human)

  • Positive control
    • Recombinant human MSK2 / RSK-B.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4)
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab71964 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    2934C1a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 38 kDa (predicted molecular weight: 86 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • Function
    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto-oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 4 antibody
    • EC 2.7.11.1 antibody
    • KS6A4_HUMAN antibody
    • Mitogen and stress activated protein kinase 2 antibody
    • Nuclear mitogen and stress activated protein kinase 2 antibody
    • Nuclear mitogen- and stress-activated protein kinase 2 antibody
    • Ribosomal protein kinase B antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 4 antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 4 antibody
    • Ribosomal protein S6 kinase alpha 4 antibody
    • Ribosomal protein S6 kinase alpha-4 antibody
    • RPS6KA4 antibody
    • RSKB antibody
    • S6K-alpha-4 antibody
    see all

Images

  • Anti-MSK2 / RSK-B antibody [2934C1a] (ab71964) at 1/100 dilution + immunising recombinant protein at 0.025 µg

    Secondary
    Sheep Anti Mouse IgG at 1/3000 dilution

    Predicted band size : 86 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

References

ab71964 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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