Overview

  • Product nameAnti-MSK2 / RSK-B antibody
    See all MSK2 / RSK-B primary antibodies
  • Description
    Rabbit polyclonal to MSK2 / RSK-B
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide from MSK2/ RSK-B (Human)

  • Positive control
    • A431 or 293T cells lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab51131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 84 kDa.

Target

  • FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto-oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 4 antibody
    • EC 2.7.11.1 antibody
    • KS6A4_HUMAN antibody
    • Mitogen and stress activated protein kinase 2 antibody
    • Nuclear mitogen and stress activated protein kinase 2 antibody
    • Nuclear mitogen- and stress-activated protein kinase 2 antibody
    • Ribosomal protein kinase B antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 4 antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 4 antibody
    • Ribosomal protein S6 kinase alpha 4 antibody
    • Ribosomal protein S6 kinase alpha-4 antibody
    • RPS6KA4 antibody
    • RSKB antibody
    • S6K-alpha-4 antibody
    see all

Anti-MSK2 / RSK-B antibody images

  • All lanes : Anti-MSK2 / RSK-B antibody (ab51131) at 1/2000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : 293T cell lysate


    Predicted band size : 84 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MSK2 / RSK-B antibody (ab51131)

This product has been referenced in:
  • Jacks KA & Koch CA Differential regulation of mitogen- and stress-activated protein kinase-1 and -2 (MSK1 and MSK2) by CK2 following UV radiation. J Biol Chem 285:1661-70 (2010). WB ; Human . Read more (PubMed: 19933278) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Epithelial, neutrophil, monocyte)
Loading amount 500000 cells
Specification Epithelial, neutrophil, monocyte
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Sep 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"