Anti-MSK2 / RSK-B (phospho T568) antibody (ab79502)

Overview

  • Product name
    Anti-MSK2 / RSK-B (phospho T568) antibody
    See all MSK2 / RSK-B primary antibodies
  • Description
    Rabbit polyclonal to MSK2 / RSK-B (phospho T568)
  • Specificity
    ab79502 detects endogenous levels of MSK2 / RSK-B only when phosphorylated at threonine 568.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human MSK2/RSK-B around the phosphorylation site of threonine 568 (MQTPPC)

  • Positive control
    • IHC: human brain tissue WB: extracts from 293 cells (treated with H2O2 at 100uM for 15mins)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purifed by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 86 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto-oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 4 antibody
    • EC 2.7.11.1 antibody
    • KS6A4_HUMAN antibody
    • Mitogen and stress activated protein kinase 2 antibody
    • Nuclear mitogen and stress activated protein kinase 2 antibody
    • Nuclear mitogen- and stress-activated protein kinase 2 antibody
    • Ribosomal protein kinase B antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 4 antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 4 antibody
    • Ribosomal protein S6 kinase alpha 4 antibody
    • Ribosomal protein S6 kinase alpha-4 antibody
    • RPS6KA4 antibody
    • RSKB antibody
    • S6K-alpha-4 antibody
    see all

Anti-MSK2 / RSK-B (phospho T568) antibody images

  • All lanes : Anti-MSK2 / RSK-B (phospho T568) antibody (ab79502) at 1/1000 dilution

    Lane 1 : extracts from 293 cells (treated with H2O2 at 100uM for 15mins)
    Lane 2 : extracts from 293 cells (treated with H2O2 at 100uM for 15mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 86 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, stained with ab79502, at 1/100 dilution, in the absence and presence of the immunising peptide

References for Anti-MSK2 / RSK-B (phospho T568) antibody (ab79502)

ab79502 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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