Overview

  • Product name
  • Description
    Rabbit polyclonal to MSL1
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence corresponding to amino acids 236-330 (QQQQLQAKEK EIEELKSERD TLLARIERME RRMQLVKKDN EKERHKLFQG YETEEREETE LSEKIKLECQ PELSETSQTL PPKPFSCGRS GKGHK) of Human MSL1v1.

  • Positive control
    • Human prostate tissue; A431 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function
    Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at 'Lys-16' which is implicated in the formation of higher-order chromatin structure.
  • Sequence similarities
    Belongs to the msl-1 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hMSL1 antibody
    • male specific lethal 1 antibody
    • male specific lethal 1 homolog 1 antibody
    • male specific lethal 1 homolog antibody
    • male specific lethal 1 like 1 antibody
    • Male-specific lethal 1 homolog antibody
    • Male-specific lethal 1-like 1 antibody
    • Male-specific lethal-1 homolog 1 antibody
    • MSL 1 antibody
    • MSL-1 antibody
    • Msl1 antibody
    • MSL1 like 1 antibody
    • MSL1-like 1 antibody
    • MSL1_HUMAN antibody
    see all

Images

  • ab121718 at 1/400 dilution staining MSL1v1 in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • ab121718 at 4 µg/ml staining MSL1v1 (green) in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence.

References

ab121718 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Treatment
transfected with Flag-MSL1 plasmid
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Sep 01 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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