This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

Applications

  • Application notesWB: Use at a 1/250 dilution. Detects a band of approximately 210 kDa (predicted molecular weight: 121 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-MSL1v1 antibody images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Anti-MSL1v1 antibody (ab35160) at 1/250 dilution + Ovary (Human) Tissue Lysate at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 121 kDa
      Observed band size : 210 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab35160 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab35160, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells.  However, this Fast-Track antibody is not yet fully characterised.  This image represents inconclusive preliminary data.

    References for Anti-MSL1v1 antibody (ab35160)

    ab35160 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - nuclear (H1299 cells)
    Loading amount 100 µg
    Specification H1299 cells
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Steven McMahon

    Verified customer

    Submitted Oct 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"