Anti-MST1/MST2 (phospho T180 + T183) antibody [EPR1467Y] (ab76323)

Overview

  • Product nameAnti-MST1/MST2 (phospho T180 + T183) antibody [EPR1467Y]
    See all MST1/MST2 primary antibodies
  • Description
    Rabbit monoclonal [EPR1467Y] to MST1/MST2 (phospho T180 + T183)
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho synthetic peptide corresponding to residues surrounding Threonine 183 of human MST1, or Threonine 180 of human MST2.

  • Positive control
    • HeLa cell lysates treated with Calyculin A.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 56 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • RelevanceThe human serine/threonine kinase, mammalian STE20 like kinase (MST), is considerably homologous to the budding yeast kinases, SPS1 and STE20, throughout their kinase domains. When stably expressed in HeLa cells, MST highly sensitizes the cells to death receptor mediated apoptosis by accelerating caspase 3 activation. These findings suggest that MST1 and MST2 play a role in apoptosis both upstream and downstream of caspase activation. MST1 is cleaved and activated by caspases during apoptosis and is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. Mammalian Sterile 20 Like 2 (MST2) is most similar to the previously identified MST1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in vitro kinase assay indicates that MST2 can phosphorylate an exogenous substrate, as well as itself, and phospho amino acid analysis indicates that it is a serine/threonine protein kinase.
    • Cellular localizationCytoplasm. Nucleus. Note: The caspase cleaved form cycles between the nucleus and cytoplasm.
    • Database links
    • Alternative names
      • DKFZp686A2068 antibody
      • FLJ90748 antibody
      • KRS1 antibody
      • KRS2 antibody
      • Mammalian STE20 like protein kinase 1 antibody
      • Mammalian STE20 like protein kinase 2 antibody
      • MST 1 antibody
      • MST 2 antibody
      • MST1 antibody
      • MST2 antibody
      • Serine/threonine kinase 3 (STE20 homolog yeast) antibody
      • Serine/threonine kinase 3 (Ste20 yeast homolog) antibody
      • Serine/threonine kinase 3 antibody
      • Serine/threonine kinase 4 antibody
      • Serine/threonine protein kinase 3 antibody
      • Serine/threonine protein kinase 4 antibody
      • Serine/threonine protein kinase Krs 1 antibody
      • Serine/threonine protein kinase Krs 2 antibody
      • STE20 like kinase MST1 antibody
      • STE20 like kinase MST2 antibody
      • STK 3 antibody
      • STK 4 antibody
      • STK3 antibody
      • STK4 antibody
      • YSK3 antibody
      see all

    Anti-MST1/MST2 (phospho T180 + T183) antibody [EPR1467Y] images

    • All lanes : Anti-MST1/MST2 (phospho T180 + T183) antibody [EPR1467Y] (ab76323) at 1/2000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with Calyculin A.

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 56 kDa
      Observed band size : 59 kDa (why is the actual band size different from the predicted?)

    References for Anti-MST1/MST2 (phospho T180 + T183) antibody [EPR1467Y] (ab76323)

    ab76323 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Hela cells transfected with human MST2)
    Loading amount 5 µg
    Specification Hela cells transfected with human MST2
    Gel Running Conditions Non-reduced Denaturing (10% SDS running gel)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Aug 16 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"