Anti-MST1/MST2 (phospho T183) antibody (ab79199)


  • Product nameAnti-MST1/MST2 (phospho T183) antibody
    See all MST1/MST2 primary antibodies
  • Description
    Rabbit polyclonal to MST1/MST2 (phospho T183)
  • Specificityab79199 detects endogenous levels of MST1/MST2 only when phosphorylated at threonine 183.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human MST1/MST2 around the phosphorylation site of threonine 183 (R-N-TP-V-I).

  • Positive control
    • Human liver carcinoma tissue. Extracts from 293 cells treated with H2O2 (100uM, 15mins).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab79199 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab79199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.


  • RelevanceThe human serine/threonine kinase, mammalian STE20 like kinase (MST), is considerably homologous to the budding yeast kinases, SPS1 and STE20, throughout their kinase domains. When stably expressed in HeLa cells, MST highly sensitizes the cells to death receptor mediated apoptosis by accelerating caspase 3 activation. These findings suggest that MST1 and MST2 play a role in apoptosis both upstream and downstream of caspase activation. MST1 is cleaved and activated by caspases during apoptosis and is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. Mammalian Sterile 20 Like 2 (MST2) is most similar to the previously identified MST1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in vitro kinase assay indicates that MST2 can phosphorylate an exogenous substrate, as well as itself, and phospho amino acid analysis indicates that it is a serine/threonine protein kinase.
  • Cellular localizationCytoplasm. Nucleus. Note: The caspase cleaved form cycles between the nucleus and cytoplasm.
  • Database links
  • Alternative names
    • DKFZp686A2068 antibody
    • FLJ90748 antibody
    • KRS1 antibody
    • KRS2 antibody
    • Mammalian STE20 like protein kinase 1 antibody
    • Mammalian STE20 like protein kinase 2 antibody
    • MST 1 antibody
    • MST 2 antibody
    • MST1 antibody
    • MST2 antibody
    • Serine/threonine kinase 3 (STE20 homolog yeast) antibody
    • Serine/threonine kinase 3 (Ste20 yeast homolog) antibody
    • Serine/threonine kinase 3 antibody
    • Serine/threonine kinase 4 antibody
    • Serine/threonine protein kinase 3 antibody
    • Serine/threonine protein kinase 4 antibody
    • Serine/threonine protein kinase Krs 1 antibody
    • Serine/threonine protein kinase Krs 2 antibody
    • STE20 like kinase MST1 antibody
    • STE20 like kinase MST2 antibody
    • STK 3 antibody
    • STK 4 antibody
    • STK3 antibody
    • STK4 antibody
    • YSK3 antibody
    see all

Anti-MST1/MST2 (phospho T183) antibody images

  • All lanes : Anti-MST1/MST2 (phospho T183) antibody (ab79199)

    Lane 1 : Extracts from 293 cells, treated with H2O2 (100uM, 15mins)
    Lane 2 : Extracts from 293 cells, treated with H2O2 (100uM, 15mins) with immunising phosphopeptide

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab79199, at 1/50 dilution, staining MST1/MST2 in human liver carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.

References for Anti-MST1/MST2 (phospho T183) antibody (ab79199)

This product has been referenced in:
  • Dixit D  et al. Chaetocin-induced ROS-mediated apoptosis involves ATM-YAP1 axis and JNK-dependent inhibition of glucose metabolism. Cell Death Dis 5:e1212 (2014). WB ; Human . Read more (PubMed: 24810048) »

See 1 Publication for this product

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Since ab79199 has not been tried on mouse samples, we do not guarantee it for this species. The guarantee applies for tested species and applications only.

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I am sorry to hear that the antibodies are not working as expected.

Your protocol looks fine to me. I wanted to ask you a few more questions:

1) Both antibodies detect a phospho-site. Under the expe...

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