Overview

  • Product name
    Anti-MST4 antibody [EP1864Y]
    See all MST4 primary antibodies
  • Description
    Rabbit monoclonal [EP1864Y] to MST4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MST4 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: HeLa cell lysate IHC-P: Human placenta tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab52491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/100000 - 1/200000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IP 1/50.
ICC 1/100 - 1/250.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

  • Function
    Mediator of cell growth. Modulates apoptosis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Golgi apparatus membrane. Localizes to the Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610018G03Rik antibody
    • EC 2.7.11.1 antibody
    • Mammalian Ste20 like protein kinase 4 antibody
    • Mammalian STE20-like protein kinase 4 antibody
    • Mammalian sterile 20 like 4 antibody
    • MASK antibody
    • MST 4 antibody
    • MST-4 antibody
    • MST3 and SOK1 related kinase antibody
    • Mst3 and SOK1-related kinase antibody
    • Mst4 antibody
    • MST4_HUMAN antibody
    • RGD1563568 antibody
    • RP23-245F8.1 antibody
    • RP6 213H19.1 antibody
    • Serine/threonine protein kinase 26 antibody
    • Serine/threonine protein kinase MASK antibody
    • Serine/threonine protein kinase MST 4 antibody
    • Serine/threonine protein kinase MST4 antibody
    • Serine/threonine-protein kinase MASK antibody
    • Serine/threonine-protein kinase MST4 antibody
    • STE20 like kinase MST 4 antibody
    • STE20 like kinase MST4 antibody
    • STE20-like kinase MST4 antibody
    • STK26 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling MST4 with purified ab52491 at 1/500. Cells were fixed with 100% methanol and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Anti-MST4 antibody [EP1864Y] (ab52491) at 1/200000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 47 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)

  • Overlay histogram showing HeLa cells stained with ab52491 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52491, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemical analysis of paraffin-embedded human placenta using ab52491 at a 1/100 dilution.
  • Anti-MST4 antibody [EP1864Y] (ab52491) at 1/20000 dilution + Recombinant human MST4 protein (ab60341) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 47 kDa


    Exposure time: 20 seconds


    Ab52491 recognizes the full length tagged recombinant MST4 protein (ab60341) which has an expected molecular weight of 72 kDa.
  • All lanes : Anti-MST4 antibody [EP1864Y] (ab52491) at 1/100000 dilution

    Lane 1 : Mouse brain tissue lysate with 5% NFDM/TBST
    Lane 2 : Rat brain tissue lysate with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 47 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds

References

This product has been referenced in:
  • Huang T  et al. MST4 Phosphorylation of ATG4B Regulates Autophagic Activity, Tumorigenicity, and Radioresistance in Glioblastoma. Cancer Cell 32:840-855.e8 (2017). IHC-P ; Human . Read more (PubMed: 29232556) »
  • Hebert AM  et al. Merlin/ERM proteins establish cortical asymmetry and centrosome position. Genes Dev 26:2709-23 (2012). WB ; Human . Read more (PubMed: 23249734) »

See all 6 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab52491.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up