• Product name
    Anti-MST4 antibody [EP1864Y]
    See all MST4 primary antibodies
  • Description
    Rabbit monoclonal [EP1864Y] to MST4
  • Tested applications
    Suitable for: ICC/IF, WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the N terminus of human Mst4

  • Positive control
    • WB: HeLa cell lysate IHC-P: Human placenta tissue
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab52491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/100000 - 1/200000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IP 1/50.
ICC 1/100 - 1/250.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.


  • Function
    Mediator of cell growth. Modulates apoptosis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Golgi apparatus membrane. Localizes to the Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610018G03Rik antibody
    • EC antibody
    • Mammalian Ste20 like protein kinase 4 antibody
    • Mammalian STE20-like protein kinase 4 antibody
    • Mammalian sterile 20 like 4 antibody
    • MASK antibody
    • MST 4 antibody
    • MST-4 antibody
    • MST3 and SOK1 related kinase antibody
    • Mst3 and SOK1-related kinase antibody
    • Mst4 antibody
    • MST4_HUMAN antibody
    • RGD1563568 antibody
    • RP23-245F8.1 antibody
    • RP6 213H19.1 antibody
    • Serine/threonine protein kinase 26 antibody
    • Serine/threonine protein kinase MASK antibody
    • Serine/threonine protein kinase MST 4 antibody
    • Serine/threonine protein kinase MST4 antibody
    • Serine/threonine-protein kinase MASK antibody
    • Serine/threonine-protein kinase MST4 antibody
    • STE20 like kinase MST 4 antibody
    • STE20 like kinase MST4 antibody
    • STE20-like kinase MST4 antibody
    • STK26 antibody
    see all

Anti-MST4 antibody [EP1864Y] images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling MST4 with purified ab52491 at 1/500. Cells were fixed with 100% methanol and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Anti-MST4 antibody [EP1864Y] (ab52491) at 1/200000 dilution + HeLa cell lysate at 10 µg

    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab52491 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52491, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemical analysis of paraffin-embedded human placenta using ab52491 at a 1/100 dilution.

  • Predicted band size : 47 kDa

    Exposure time : 20 secondsAb52491 recognizes the full length tagged recombinant MST4 protein (ab60341) which has an expected molecular weight of 72 kDa.

References for Anti-MST4 antibody [EP1864Y] (ab52491)

This product has been referenced in:
  • Fidalgo M  et al. Adaptor protein cerebral cavernous malformation 3 (CCM3) mediates phosphorylation of the cytoskeletal proteins ezrin/radixin/moesin by mammalian Ste20-4 to protect cells from oxidative stress. J Biol Chem 287:11556-65 (2012). Read more (PubMed: 22291017) »
  • Hebert AM  et al. Merlin/ERM proteins establish cortical asymmetry and centrosome position. Genes Dev 26:2709-23 (2012). WB ; Human . Read more (PubMed: 23249734) »

See all 5 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab52491.
Please use the links above to contact us or submit feedback about this product.


Sign up