Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling MST4 with purified ab52491 at 1/500. Cells were fixed with 100% methanol and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Western blot - MST4 antibody [EP1864Y] (ab52491)
Anti-MST4 antibody [EP1864Y] (ab52491) at 1/200000 dilution + HeLa cell lysate at 10 µg
Overlay histogram showing HeLa cells stained with ab52491 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52491, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunohistochemical analysis of paraffin-embedded human placenta using ab52491 at a 1/100 dilution.
Western blot - Anti-MST4 antibody [EP1864Y] (ab52491)
Predicted band size : 47 kDa
Exposure time : 20 secondsAb52491 recognizes the full length tagged recombinant MST4 protein (ab60341) which has an expected molecular weight of 72 kDa.
References for Anti-MST4 antibody [EP1864Y] (ab52491)
This product has been referenced in:
Fidalgo M et al. Adaptor protein cerebral cavernous malformation 3 (CCM3) mediates phosphorylation of the cytoskeletal proteins ezrin/radixin/moesin by mammalian Ste20-4 to protect cells from oxidative stress. J Biol Chem287:11556-65 (2012).
Read more (PubMed: 22291017) »
Hebert AM et al. Merlin/ERM proteins establish cortical asymmetry and centrosome position. Genes Dev26:2709-23 (2012).
Read more (PubMed: 23249734) »