Anti-MSX1 antibody (ab49153)

Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/62500.

Target

  • FunctionActs as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity.
  • Tissue specificityExpressed in the developing nail bed mesenchyme.
  • Involvement in diseaseDefects in MSX1 are the cause of tooth agenesis selective type 1 (STHAG1) [MIM:106600]. A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). Tooth agenesis selective type 1 can be associated with orofacial cleft in some patients.
    Note=MSX1 is deleted in some patients with Wolf-Hirschhorn syndrome (WHS). WHS results from sub-telomeric deletions in the short arm of chromosome 4.
    Defects in MSX1 are the cause of Witkop syndrome (WITS) [MIM:189500]. WITS is a form of ectodermal dyslasia also called tooth-and-nail syndrome or dysplasia of nails with hypodontia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. Witkop syndrome is characterized by abnormalities largely limited largely to teeth (some of which are missing) and nails (which are poorly formed early in life, especially toenails). This condition is distinguished from anhidrotic ectodermal dysplasia by autosomal dominant inheritance and little involvement of hair and sweat glands. The teeth are not as severely affected.
    Defects in MSX1 are the cause of non-syndromic orofacial cleft type 5 (OFC5) [MIM:608874]; also called non-syndromic cleft lip with or without cleft palate 5. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • Sequence similaritiesBelongs to the Msh homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Sumoylated by PIAS1, desumoylated by SENP1.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA675338 antibody
    • AI324650 antibody
    • Homeobox 7 antibody
    • Homeobox protein Hox-7 antibody
    • Homeobox protein MSX 1 antibody
    • Homeobox protein MSX-1 antibody
    • Homeobox protein MSX1 antibody
    • Homeobox, msh like 1 antibody
    • Homeobox, msh-like 1 antibody
    • HOX 7 antibody
    • Hox 7.1 antibody
    • Hox-7 antibody
    • HOX7 antibody
    • Hox7.1 antibody
    • HYD 1 antibody
    • HYD1 antibody
    • msh (Drosophila) homeo box homolog 1 (formerly homeo box 7) antibody
    • Msh antibody
    • msh homeo box 1 antibody
    • msh homeo box homolog 1 antibody
    • Msh homeobox 1 antibody
    • Msh homeobox 1 like protein antibody
    • Msh homeobox 1-like protein antibody
    • msh homeobox homolog 1 (Drosophila) antibody
    • msh homeobox homolog 1 antibody
    • MSH, Drosophila, Homolog of, 1 antibody
    • MSX 1 antibody
    • MSX1 antibody
    • MSX1_HUMAN antibody
    • Muscle segment homeobox antibody
    • Muscle segment homeobox, Drosophila, Homolog of, 1 antibody
    • OFC5 antibody
    • OTTHUMP00000115387 antibody
    • STHAG1 antibody
    see all

Anti-MSX1 antibody images

  • Anti-MSX1 antibody (ab49153) at 2 µg/ml + Fetal kidney lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/50,000 - 1/100,000

    Predicted band size : 31 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling MSX1 with ab49153 at 4-8µg/ml.

References for Anti-MSX1 antibody (ab49153)

ab49153 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.5% triton
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Sueli Marques

Verified customer

Submitted Dec 22 2015

Thanks for your call today, and I'm sorry to hear that we could not get these antibodies working for you. I have issued credit notes for the orders. Since these orders were placed by credit card, the purchase prices will be refunded directly back to yo...

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I have taken a look at your images and have the following suggestions to make: 1.) Because a lot of the background in your no primary/ no secondary image is nuclear, it is possible that the DAPI signal is being detected by your 488 filters.  The spectr...

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Thanks for your call yesterday and for sending the images. Please keep me posted on the results of the staining today. I am still a little stumped as to why the staining would be more intense in the no primary control, even if there were contamination ...

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Thank you for your call yesterday and for sending the emails with your images. I have a few suggestions but I just want to check that the "no primary" control (incubated with just the secondary antibody) is not showing any staining at all? As we discus...

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Thank you for sending the images. I hope that my colleague answered all of your questions yesterday regarding your replacement slides and antibody, and I apologize that I had not previously sent this information to you. We expect that both of these ite...

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