Anti-Msx2/Hox8 antibody - N-terminal (ab190070)
Key features and details
- Rabbit polyclonal to Msx2/Hox8 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Msx2/Hox8 antibody - N-terminal
See all Msx2/Hox8 primary antibodies -
Description
Rabbit polyclonal to Msx2/Hox8 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Msx2/Hox8 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). NP_002440.2.
Database link: P35548 -
Positive control
- Human skin tissue; 293 cell lysates transiently transfected with the Msx2/Hox8 gene.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 29 kDa.
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IHC-P |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/1000. Predicted molecular weight: 29 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antogonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter. -
Involvement in disease
Defects in MSX2 are the cause of parietal foramina 1 (PFM1) [MIM:168500]; also known as foramina parietalia permagna (FPP). PFM1 is an autosomal dominant disease characterized by oval defects of the parietal bones caused by deficient ossification around the parietal notch, which is normally obliterated during the fifth fetal month.
Defects in MSX2 are the cause of parietal foramina with cleidocranial dysplasia (PFMCCD) [MIM:168550]; also known as cleidocranial dysplasia with parietal foramina. PFMCCD combines skull defects in the form of enlarged parietal foramina and deficient ossification of the clavicles.
Defects in MSX2 are the cause of craniosynostosis type 2 (CRS2) [MIM:604757]; also known as craniosynostosis Boston-type (CSB). CRS2 is an autosomal dominant disorder characterized by the premature fusion of calvarial sutures. The craniosynostosis phenotype is either fronto-orbital recession, or frontal bossing, or turribrachycephaly, or cloverleaf skull. Associated features include severe headache, high incidence of visual problems (myopia or hyperopia), and short first metatarsals. Intelligence is normal. -
Sequence similarities
Belongs to the Msh homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4488 Human
- Omim: 123101 Human
- SwissProt: P35548 Human
- Unigene: 89404 Human
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Alternative names
- CRS 2 antibody
- CRS2 antibody
- FPP antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling Msx2/Hox8 with ab190070 at 10 µg/ml.
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All lanes : Anti-Msx2/Hox8 antibody - N-terminal (ab190070) at 1/1000 dilution
Lane 1 : 293 cell lysate, non-transfected
Lane 2 : 293 cell lysate, transiently transfected with Msx2/Hox8 gene
Lysates/proteins at 2 µg per lane.
Predicted band size: 29 kDa
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab190070 has been referenced in 1 publication.
- Kero D et al. Regulation of proliferation in developing human tooth germs by MSX homeodomain proteins and cyclin-dependent kinase inhibitor p19INK4d. Organogenesis 13:141-155 (2017). PubMed: 28933666