Overview

  • Product nameAnti-MSY2/YBOX2 antibody
    See all MSY2/YBOX2 primary antibodies
  • Description
    Rabbit polyclonal to MSY2/YBOX2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (EPQKGGGAGG GGGAASGPAA GTPSAPGSRT PGNPATAVSG TPAPPARSQA) of human MSY2/YBOX2 (NP_057066).

  • Positive control
    • Fetal lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

Target

Anti-MSY2/YBOX2 antibody images

  • Anti-MSY2/YBOX2 antibody (ab82963) at 1 µg/ml + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

References for Anti-MSY2/YBOX2 antibody (ab82963)

ab82963 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 30000 cells
Specification 293T
Gel Running Conditions Reduced Denaturing (4-20% TrisGlycine)
Blocking step 5% Milk, 1% BSAS as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Feb 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"