Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)

Overview

  • Product name
    Anti-MT-CYB antibody [5B3-6E3] - N-terminal
    See all MT-CYB primary antibodies
  • Description
    Mouse monoclonal [5B3-6E3] to MT-CYB - N-terminal
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MT-CYB (N terminal).
    Database link: P00156

  • Positive control
    • WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn); Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn).

Properties

Applications

Our Abpromise guarantee covers the use of ab219823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.05 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 43 kDa).

Target

  • Function
    Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
  • Involvement in disease
    Defects in MT-CYB are a rare cause of mitochondrial dysfunction underlying different myopathies. They include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy (HCM), and sporadic mitochondrial myopathy (MM). In mitochondrial myopathy, exercise intolerance is the predominant symptom. Additional features include lactic acidosis, muscle weakness and/or myoglobinuria. Defects in MTCYB are also found in cases of exercise intolerance accompanied by deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, epilepsy (multisystem disorder).
    Cardiomyopathy, infantile histiocytoid
    Leber hereditary optic neuropathy
  • Sequence similarities
    Belongs to the cytochrome b family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COB antibody
    • Complex III subunit 3 antibody
    • Complex III subunit III antibody
    • CYB_HUMAN antibody
    • CYTB antibody
    • Cytochrome b antibody
    • Cytochrome b c1 complex subunit 3 antibody
    • Cytochrome b-c1 complex subunit 3 antibody
    • Mitochondrially encoded cytochrome b antibody
    • MT-CYB antibody
    • MTCYB antibody
    • Ubiquinol cytochrome c reductase complex cytochrome b subunit antibody
    • Ubiquinol-cytochrome-c reductase complex cytochrome b subunit antibody
    see all

Images

  • All lanes : Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823) at 0.1 µg/ml

    Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
    Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-labeled Goat-anti-mouse IgG
    Developed using the ECL technique

    Predicted band size : 43 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

    Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

  • All lanes : Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823) at 2 µg/ml

    Lane 1 : Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn)
    Lane 2 : Whole cell extract HDFn-Rho0 cells depleted of mtDNA by long-term culture in the presence of ethidium bromide

    Lysates/proteins at 15 µg per lane.

    Secondary
    HRP-labeled Goat-anti-mouse IgG
    Developed using the ECL technique

    Predicted band size : 43 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

    Proteins were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

References

ab219823 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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