Overview

  • Product name
  • Description
    Rabbit polyclonal to MT-CYB
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region within internal amino acids 207-237 of Human MT-CYB.

  • Positive control
    • HL60 cell line lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab103405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 43 kDa.

Target

  • Function
    Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
  • Involvement in disease
    Defects in MT-CYB are a rare cause of mitochondrial dysfunction underlying different myopathies. They include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy (HCM), and sporadic mitochondrial myopathy (MM). In mitochondrial myopathy, exercise intolerance is the predominant symptom. Additional features include lactic acidosis, muscle weakness and/or myoglobinuria. Defects in MTCYB are also found in cases of exercise intolerance accompanied by deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, epilepsy (multisystem disorder).
    Cardiomyopathy, infantile histiocytoid
    Leber hereditary optic neuropathy
  • Sequence similarities
    Belongs to the cytochrome b family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COB antibody
    • Complex III subunit 3 antibody
    • Complex III subunit III antibody
    • CYB_HUMAN antibody
    • CYTB antibody
    • Cytochrome b antibody
    • Cytochrome b c1 complex subunit 3 antibody
    • Cytochrome b-c1 complex subunit 3 antibody
    • Mitochondrially encoded cytochrome b antibody
    • MT-CYB antibody
    • MTCYB antibody
    • Ubiquinol cytochrome c reductase complex cytochrome b subunit antibody
    • Ubiquinol-cytochrome-c reductase complex cytochrome b subunit antibody
    see all

Images

  • Anti-MT-CYB antibody (ab103405) at 1/100 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 43 kDa

References

ab103405 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Cell lysate - other (HeLa and HeLa rho-0)
Loading amount
13 µg
Specification
HeLa and HeLa rho-0
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

Submitted Jul 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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