Anti-MT-CYB antibody (ab81215)

Overview

  • Product name
  • Description
    Rabbit polyclonal to MT-CYB
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (TPMRKINPLM KLINHSFIDL PTPSNISAWW NFGSLLGACL ILQITTGLFL) of Human MT-CYB (NP_536855).

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

  • Function
    Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
  • Involvement in disease
    Defects in MT-CYB are a rare cause of mitochondrial dysfunction underlying different myopathies. They include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy (HCM), and sporadic mitochondrial myopathy (MM). In mitochondrial myopathy, exercise intolerance is the predominant symptom. Additional features include lactic acidosis, muscle weakness and/or myoglobinuria. Defects in MTCYB are also found in cases of exercise intolerance accompanied by deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, epilepsy (multisystem disorder).
    Cardiomyopathy, infantile histiocytoid
    Leber hereditary optic neuropathy
  • Sequence similarities
    Belongs to the cytochrome b family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COB antibody
    • Complex III subunit 3 antibody
    • Complex III subunit III antibody
    • CYB_HUMAN antibody
    • CYTB antibody
    • Cytochrome b antibody
    • Cytochrome b c1 complex subunit 3 antibody
    • Cytochrome b-c1 complex subunit 3 antibody
    • Mitochondrially encoded cytochrome b antibody
    • MT-CYB antibody
    • MTCYB antibody
    • Ubiquinol cytochrome c reductase complex cytochrome b subunit antibody
    • Ubiquinol-cytochrome-c reductase complex cytochrome b subunit antibody
    see all

Images

  • Anti-MT-CYB antibody (ab81215) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

References

ab81215 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa wt vs HeLa rho-0)
Loading amount
70 µg
Specification
HeLa wt vs HeLa rho-0
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 02 2012

Thanks for your patience.  I have place the order for ab103405 (order#******), you should hopefully have it by the 20th Oct. If there is anything else I can do, just give me a shout. You have a great weekend too.

Thank you forcontacting Abcam about ab81215, anti-MT-CYB antibody I am sorry to hear that you are experiencing problems with this antibody.  It is covered under our Abpromise, which guarantees that it will work on human tissue in western blot. I kn...

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