Anti-MT-ND5 antibody (ab83985)

Overview

  • Product nameAnti-MT-ND5 antibody
    See all MT-ND5 primary antibodies
  • Description
    Rabbit polyclonal to MT-ND5
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 38-87 (SIVASTFIIS LFPTTMFMCL DQEVIISNWH WATTQTTQLS LSFKLDYFSM) of Human MT-ND5 (Swiss-Prot P03915).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in diseaseDefects in MT-ND5 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-ND5 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
    Defects in MT-ND5 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
    Defects in MT-ND5 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
  • Sequence similaritiesBelongs to the complex I subunit 5 family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Complex I, subunit ND5 antibody
    • EC 1.6.5.3 antibody
    • Mitochondrially encoded NADH dehydrogenase 5 antibody
    • MT ND5 antibody
    • MT-ND5 antibody
    • MTND 5 antibody
    • MTND5 antibody
    • NAD5 antibody
    • NADH dehydrogenase subunit 5 (complex I) antibody
    • NADH dehydrogenase subunit 5 antibody
    • NADH ubiquinone oxidoreductase , subunit ND5 antibody
    • NADH ubiquinone oxidoreductase chain 5 antibody
    • NADH-ubiquinone oxidoreductase chain 5 antibody
    • NADH5 antibody
    • ND5 antibody
    • NU5M_HUMAN antibody
    see all

Anti-MT-ND5 antibody images

  • Anti-MT-ND5 antibody (ab83985) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 67 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab83985 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83985, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MT-ND5 antibody (ab83985)

ab83985 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - other (Mitochondrial fractions of HeLa WT and HeLa rho-0)
Loading amount 26 µg
Specification Mitochondrial fractions of HeLa WT and HeLa rho-0
Gel Running Conditions Reduced Denaturing (Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Aug 21 2012

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We generally look for a minimum of 85% level of homology between an immunogen sequence and a target sequence before recommending an antibody.

Since there is only a 65% level of homology between mouse an...

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