• Product name
  • Description
    Rabbit polyclonal to MT-ND5
  • Host species
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide between 550~579 amino acids selected from the C terminal region of Human MT-ND5 conjugated to KLH.

  • Positive control
    • WB: CEM cell lysate. IHC-P: Human normal cerebral cortex.



Our Abpromise guarantee covers the use of ab92624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 67 kDa.
ELISA 1/1000.
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in disease
    Defects in MT-ND5 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-ND5 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
    Defects in MT-ND5 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
    Defects in MT-ND5 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
  • Sequence similarities
    Belongs to the complex I subunit 5 family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Complex I, subunit ND5 antibody
    • EC antibody
    • Mitochondrially encoded NADH dehydrogenase 5 antibody
    • MT ND5 antibody
    • MT-ND5 antibody
    • MTND 5 antibody
    • MTND5 antibody
    • NAD5 antibody
    • NADH dehydrogenase subunit 5 (complex I) antibody
    • NADH dehydrogenase subunit 5 antibody
    • NADH ubiquinone oxidoreductase , subunit ND5 antibody
    • NADH ubiquinone oxidoreductase chain 5 antibody
    • NADH-ubiquinone oxidoreductase chain 5 antibody
    • NADH5 antibody
    • ND5 antibody
    • NU5M_HUMAN antibody
    see all


  • IHC image of MT-ND5 staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92624, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-MT-ND5 antibody (ab92624) at 1/1000 dilution + CEM whole cell lysate at 35 µg

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 67 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-MT-ND5 antibody (ab92624) at 1/100 dilution

    All lanes : Human Cerebral Cortex

    Lysates/proteins at 20 µg per lane.

    All lanes : Donkey polyclonal to rabbit IgG conjugated to IRDye 800CW at 1/500 dilution

    Performed under reducing conditions.

    Predicted band size: 67 kDa

    Exposure time: 5 minutes

    Detection method: Odyssey system

    See Abreview


This product has been referenced in:
  • Li HB  et al. Mitochondrial Ribosomal Protein L10 Associates with Cyclin B1/Cdk1 Activity and Mitochondrial Function. DNA Cell Biol 35:680-690 (2016). WB ; Human . Read more (PubMed: 27726420) »
  • Wang M  et al. A deafness-associated tRNAAsp mutation alters the m1G37 modification, aminoacylation and stability of tRNAAsp and mitochondrial function. Nucleic Acids Res 44:10974-10985 (2016). WB ; Human . Read more (PubMed: 27536005) »

See all 4 Publications for this product

Customer reviews and Q&As

Western blot
Human Cell lysate - other (Mitochondrial fractions of HeLa WT and HeLa rho-0)
Loading amount
26 µg
Mitochondrial fractions of HeLa WT and HeLa rho-0
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Oct 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Tissue lysate - whole (Frontal cortex)
Loading amount
20 µg
Frontal cortex
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. Joseph Bateman

Verified customer

Submitted Oct 19 2012

Thank you for contacting Abcam.

We generally look for a minimum of 85% level of homology between an immunogen sequence and a target sequence before recommending an antibody.

Since there is only a 65% level of homology between mouse an...

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