Anti-mtRNA polymerase antibody (ab32954)

Overview

• Product nameAnti-mtRNA polymerase antibodySee all mtRNA polymerase primary antibodies ...
• Description
  Rabbit polyclonal to mtRNA polymerase
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  
  Predicted to work with: Human
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human mtRNA polymerase.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab32986.)

• Positive controlThis antibody gave a positive signal within WB in the following whole cell lysates: HeLa; HEK293: Caco2. This antibody also gave a positive signal in stained HeLa cells within Immunofluorescence.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Nucleotide metabolism

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial Biogenesis

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionDNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.
• Sequence similaritiesBelongs to the phage and mitochondrial RNA polymerase family.
• Cellular localizationMitochondrion.

Target information above from: UniProt accession O00411 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5442 Human
  • Omim: 601778 Human
  • SwissProt: O00411 Human
  • Unigene: 254113 Human

• Alternative names
  DNA-directed RNA polymerase antibodyDNA-directed RNA polymerase mitochondrial antibodyh-mtRPOL antibody

  mitochondrial antibodyMTRNAP antibodyMTRPOL antibodyPOLRMT antibodyRPOM antibodyRPOM_HUMAN antibody

  see all

Anti-mtRNA polymerase antibody images

• 

  Immunocytochemistry/ Immunofluorescence - mtRNA polymerase antibody (ab32954)
  ICC/IF image of ab32954 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab32954, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4%PFA fixed Hek293 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
• 

  Western blot - Anti-mtRNA polymerase antibody (ab32954)
  All lanes : Anti-mtRNA polymerase antibody (ab32954) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
  Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab52256)
  Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab76828)
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 139 kDa
  
  
  Exposure time : 20 minutes
• 

  Western blot - Anti-mtRNA polymerase antibody (ab32954)
  All lanes : Anti-mtRNA polymerase antibody (ab32954) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  
  Predicted band size : 139 kDa
  Observed band size : 145 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.