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mtRNA polymerase protein (Tagged-His Tag) (ab91945)

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Overview

  • Product namemtRNA polymerase protein (Tagged-His Tag)See all mtRNA polymerase proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1051-1230 (180 amino acids) of Human mtRNA polymerase with an N-terminal His tag; MWt 21 kDa (UniProt O00411).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 79 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesQHWLTESARLISHMGSVVEWVTPLGVPVIQPYRLDSKVKQ IGGGIQSIT YTHNGDISRKPNTRKQKNGFPPNFIHSLD SSHMMLTALHCYRKGLTFVS VHDCYWTHAADVSVMNQV CREQFVRLHSEPILQDLSRFLVKRFCSEPQK ILEASQL KETLQAVPKPGAFDLEQVKRSTYFFS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91945 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        DNA-directed RNA polymeraseDNA-directed RNA polymerase mitochondrialh-mtRPOL
        mitochondrialMTRNAPMTRPOLPOLRMTRPOMRPOM_HUMAN
      see all
  • FunctionDNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.
  • Sequence similaritiesBelongs to the phage and mitochondrial RNA polymerase family.
  • Cellular localizationMitochondrion.

    • Target information above from: UniProt accession O00411 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    mtRNA polymerase protein (Tagged-His Tag) images

    • SDS-PAGE - mtRNA polymerase protein (Tagged-His Tag) (ab91945)
      SDS-PAGE - mtRNA polymerase protein (Tagged-His Tag) (ab91945)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for mtRNA polymerase protein (Tagged-His Tag) (ab91945)

    ab91945 has not yet been referenced specifically in any publications.

    Publishing research using ab91945 ? Please let us know so that we can cite the reference in this datasheet

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