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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Anti-mtTFA antibody [18G102B2E11] (ab119684)
MS Catalog No. MS993
Product partner
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab119684? Please let us know so that we can cite the reference in this datasheet
ab119684 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [18G102B2E11] (ab119684)](/ps/datasheet/images/119/ab119684/mtTFA-Primary-antibodies-ab119684-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [18G102B2E11] (ab119684)
Immunocytochemistry image of ab119684 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (18G102B2E11, 1µg/ml) for 2h at room temperature. The secondary antibody was (green) 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). mtTFA localizes to the mitochondria.
![Flow Cytometry - Anti-mtTFA antibody [18G102B2E11] (ab119684)](/ps/datasheet/images/119/ab119684/mtTFA-Primary-antibodies-ab119684-3.jpg)
Flow Cytometry - Anti-mtTFA antibody [18G102B2E11] (ab119684)
HeLa cells were stained with 2 µg/mL mtTFA antibody (ab119684) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
![Western blot - Anti-mtTFA antibody [18G102B2E11] (ab119684)](/ps/datasheet/images/119/ab119684/mtTFA-Primary-antibodies-ab119684-5.jpg)
Western blot - Anti-mtTFA antibody [18G102B2E11] (ab119684)
All lanes : Anti-mtTFA antibody [18G102B2E11] (ab119684) at 1 µg/ml
Lane 1 : recombinant mtTFA (ab103784) at 0.025 µg
Lane 2 : HeLa whole cell lysate at 30 µg
Secondary
HRP-conjugated Goat polyclonal to mouse IgG at 1/5000 dilution
Predicted band size : 29 kDa
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