![Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [18G102B2E11] (ab119684) image](#mtTFA-Primary-antibodies-ab119684-2.jpg)
![Flow Cytometry - Anti-mtTFA antibody [18G102B2E11] (ab119684) image](#mtTFA-Primary-antibodies-ab119684-3.jpg)
![Western blot - Anti-mtTFA antibody [18G102B2E11] (ab119684) image](#mtTFA-Primary-antibodies-ab119684-5.jpg)
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Product name
Anti-mtTFA antibody [18G102B2E11]
See all mtTFA products (7) ...
Description
Mouse monoclonal [18G102B2E11] to mtTFA
Tested applications
WB, In-Cell ELISA, ICC, Flow Cytmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Human, full-length (minus the N-terminal mitochondrial targeting sequence)
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab119684 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
Clonality
Monoclonal
Clone number
18G102B2E11
Isotype
IgG2b
Light chain type
kappa
Research areas
Metabolism >> Mitochondrial Metabolism >> Mitochondrial markers
Metabolism >> Metabolic signalling pathways >> Nucleotide metabolism >> Molecular processes >> mitochondrial transcription
Metabolism >> Mitochondrial Metabolism >> Mitochondrial Biogenesis
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab119684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 29 kDa.
In-Cell ELISA: Use a concentration of 1 µg/ml
ICC: Use a concentration of 1 µg/ml
Flow Cyt: Use a concentration of 1 µg/ml
Target
Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
Sequence similarities
Contains 2 HMG box DNA-binding domains.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionQ00059
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
- MtTF1 antibody
see all
Database links
- Entrez Gene: 7019 Human
- Omim: 600438 Human
- SwissProt: Q00059 Human
- Unigene: 642966 Human
Anti-mtTFA antibody [18G102B2E11] images:
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [18G102B2E11] (ab119684)
![Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [18G102B2E11] (ab119684)](/ps/datasheet/images/119/ab119684/mtTFA-Primary-antibodies-ab119684-2.jpg)
Immunocytochemistry image of ab119684 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (18G102B2E11, 1µg/ml) for 2h at room temperature. The secondary antibody was (green) 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). mtTFA localizes to the mitochondria.
Flow Cytometry - Anti-mtTFA antibody [18G102B2E11] (ab119684)
![Flow Cytometry - Anti-mtTFA antibody [18G102B2E11] (ab119684)](/ps/datasheet/images/119/ab119684/mtTFA-Primary-antibodies-ab119684-3.jpg)
HeLa cells were stained with 2 µg/mL mtTFA antibody (ab119684) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Western blot - Anti-mtTFA antibody [18G102B2E11] (ab119684)
![Western blot - Anti-mtTFA antibody [18G102B2E11] (ab119684)](/ps/datasheet/images/119/ab119684/mtTFA-Primary-antibodies-ab119684-5.jpg)
All lanes : Anti-mtTFA antibody [18G102B2E11] (ab119684) at 1 µg/ml
Lane 1 : recombinant mtTFA (ab103784) at 0.025 µg
Lane 2 : HeLa whole cell lysate at 30 µg
Secondary
HRP-conjugated Goat polyclonal to mouse IgG at 1/5000 dilution
Predicted band size : 29 kDa
References for Anti-mtTFA antibody [18G102B2E11] (ab119684)
ab119684 has not yet been referenced specifically in any publications.
Publishing research using ab119684? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"