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Product name
mtTFA protein
See all mtTFA products (2) ...
Protein description
Recombinant full length Human mtTFA (amino acids 43-246) with N terminal His tag; 225 amino acids with tag, MWt 26.6 kDa inclusive of tag. NP_003192. UniProt Q00059.
Uniprot accession
Q00059
Molecular weight
26.600kDa inclusive of tags
Protein length
204 amino acids
Expression host
E. coli
Properties
Purity
> 85% by SDS-PAGE
Purification notes
ab103784 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
Mass spectrometry
MALDI-TOF
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 20% Glycerol, 0.2M Sodium chloride, 5mM DTT, 20mM Tris HCl, pH 8.0
Concentration
Concentration information loading...
Sequence
MGSSHHHHHHSSGLVPRGSHMSSVLASCPKKPVSSYLRFS KEQLPIFKAQNPDAKTTELIRRIAQRWRELPDSKKKIYQD AYRAEWQVYKEEISRFKEQLTPSQIMSLEKEIMDKHLKRK AMTKKKELTLLGKPKRPRSAYNVYVAERFQEAKGDSPQEK LKTVKENWKNLSDSEKELYIQHAKEDETRYHNEMKSWEEQ MIEVGRKDLLRRTIKKQRKYGAEEC
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Nucleotide metabolism >> Molecular processes >> Mitochondrial transcription
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial Biogenesis
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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SDS-PAGE - mtTFA protein (ab103784)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab103784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
MS: Use at an assay dependent dilution.
Application notes
This peptide can be used with studies using ab123455.
Protein info
Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
Sequence similarities
Contains 2 HMG box DNA-binding domains.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionQ00059
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
mtTFA protein images:
References for mtTFA protein (ab103784)
ab103784 has not yet been referenced specifically in any publications.
Publishing research using ab103784? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"