Overview

  • Product name
  • Description
    Rabbit polyclonal to MTA1
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis, Platypus
  • Immunogen

    A synthetic peptide corresponding to a region between residue 665 and the C-terminus (residue 715) of MTA1 (NP_004680.1).

  • Positive control
    • Whole cell lysate from Hela cells, NIH3T3 cells and 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab71153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 35, 81 kDa (predicted molecular weight: 81 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    May be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER.
  • Tissue specificity
    Widely expressed. High expression in brain, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual.
  • Sequence similarities
    Contains 1 BAH domain.
    Contains 1 ELM2 domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 SANT domain.
  • Developmental stage
    Highly expressed in metastatic cells.
  • Domain
    Isoform Short contains a Leu-Arg-Ile-Leu-Leu motif (ER binding motif).
  • Cellular localization
    Cytoplasm and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Metastasis associated 1 antibody
    • Metastasis associated gene 1 antibody
    • Metastasis associated gene 1 protein antibody
    • Metastasis associated protein antibody
    • Metastasis associated protein MTA 1 antibody
    • Metastasis associated protein MTA1 antibody
    • Metastasis-associated protein MTA1 antibody
    • MTA 1 antibody
    • MTA1 antibody
    • MTA1_HUMAN antibody
    see all

Anti-MTA1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling MTA1 with ab71153 at 1/1000 (0.2 µg/ml). Detection: DAB.
  • All lanes : Anti-MTA1 antibody (ab71153) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 81 kDa
    Observed band size : 81 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of MTA1
    Lane 1: ab71153 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Subsequent WB detection was performed using 1 µg/ml ab71153.
    Chemiluminescence with an exposure time of 1 second.
  • ICC/IF image of ab71153 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MTA1 antibody (ab71153)

This product has been referenced in:
  • Ma K  et al. MTA1 promotes epithelial to mesenchymal transition and metastasis in non-small-cell lung cancer. Oncotarget 8:38825-38840 (2017). IHC . Read more (PubMed: 28418915) »
  • Liu T  et al. Hypoxia-induced MTA1 promotes MC3T3 osteoblast growth but suppresses MC3T3 osteoblast differentiation. Eur J Med Res 20:10 (2015). WB . Read more (PubMed: 25644400) »

See all 5 Publications for this product

Product Wall

Application
ChIP
Sample
Human Cell lysate - nuclear (Jurkat cells)
Negative control
ChIP with rabbit IgG qPCR on GAPDH promoter
Specification
Jurkat cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Username

Dr. Marion Dubuissez

Verified customer

Submitted Nov 03 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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