Overview

  • Product name
    Anti-MTA1 antibody [MTA1-213]
    See all MTA1 primary antibodies
  • Description
    Mouse monoclonal [MTA1-213] to MTA1
  • Tested applications
    Suitable for: WB, ELISA, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Synthetic peptide:

    KSYPTKVRLIRGGSLP

    , corresponding to C terminal amino acids 626-641 of Human MTA1

  • Positive control
    • HeLa cell nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab50263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 81 kDa.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    May be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER.
  • Tissue specificity
    Widely expressed. High expression in brain, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual.
  • Sequence similarities
    Contains 1 BAH domain.
    Contains 1 ELM2 domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 SANT domain.
  • Developmental stage
    Highly expressed in metastatic cells.
  • Domain
    Isoform Short contains a Leu-Arg-Ile-Leu-Leu motif (ER binding motif).
  • Cellular localization
    Cytoplasm and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Metastasis associated 1 antibody
    • Metastasis associated gene 1 antibody
    • Metastasis associated gene 1 protein antibody
    • Metastasis associated protein antibody
    • Metastasis associated protein MTA 1 antibody
    • Metastasis associated protein MTA1 antibody
    • Metastasis-associated protein MTA1 antibody
    • MTA 1 antibody
    • MTA1 antibody
    • MTA1_HUMAN antibody
    see all

References

This product has been referenced in:
  • Dubuissez M  et al. Protein Kinase C-Mediated Phosphorylation of BCL11B at Serine 2 Negatively Regulates Its Interaction with NuRD Complexes during CD4+ T-Cell Activation. Mol Cell Biol 36:1881-98 (2016). ChIP . Read more (PubMed: 27161321) »

See 1 Publication for this product

Customer reviews and Q&As

Application
ChIP
Sample
Human Cell lysate - nuclear (Jurkat cells)
Negative control
ChIP with IgG qPCR on GAPDH promoter
Specification
Jurkat cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
qPCR on IL2 promoter
Username

Dr. Marion Dubuissez

Verified customer

Submitted Nov 03 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up