Overview

  • Product nameAnti-MTAP antibody
    See all MTAP primary antibodies
  • Description
    Mouse monoclonal to MTAP
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-155 of Human MTAP

Properties

Applications

Our Abpromise guarantee covers the use of ab55517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 31 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPlays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the PNP/MTAP phosphorylase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' methylthioadenosine phosphorylase antibody
    • 5''-methylthioadenosine phosphorylase antibody
    • BDMF antibody
    • c86fus antibody
    • DMSFH antibody
    • DMSMFH antibody
    • Epididymis luminal protein 249 antibody
    • HEL 249 antibody
    • LGMBF antibody
    • MeSAdo phosphorylase antibody
    • Methylthioadenosine phosphorylase antibody
    • MSAP antibody
    • MTA phosphorylase antibody
    • MTAP antibody
    • MTAP_HUMAN antibody
    • MTAPase antibody
    • S methyl 5 thioadenosine phosphorylase antibody
    • S methyl 5' thioadenosine phosphorylase antibody
    • S-methyl-5''-thioadenosine phosphorylase antibody
    see all

Anti-MTAP antibody images



  • Predicted band size : 31 kDa
    MTAP antibody (ab55517) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55517 at 10 ug/ml staining MTAP in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing HeLa cells stained with ab55517 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55517, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-MTAP antibody (ab55517)

This product has been referenced in:
  • Czech B  et al. Expression and function of methylthioadenosine phosphorylase in chronic liver disease. PLoS One 8:e80703 (2013). WB, IHC-P ; Human . Read more (PubMed: 24324622) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"