Overview

  • Product nameAnti-MTBP antibody
    See all MTBP primary antibodies
  • Description
    Rabbit polyclonal to MTBP
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Synthetic peptide:

    GAVECFEEEDSNSRESLS

    conjugated to KLH, corresponding to amino acids 122-139 of Human MTBP (NP_071328.2).

  • Positive control
    • Human thymus and tonsil tissues.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 102 kDa.Can be blocked with MTBP peptide (ab6235).
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

Target

  • FunctionInhibits cell migration in vitro and suppresses the invasive behavior of tumor cells (By similarity). May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation.
  • Sequence similaritiesBelongs to the MTBP family.
  • Information by UniProt
  • Database links
  • Alternative names
    • hMTBP antibody
    • MDM 2 binding protein antibody
    • MDM 2 BP antibody
    • MDM2 (mouse double minute 2) binding protein 104kD antibody
    • MDM2 (mouse double minute 2) binding protein antibody
    • MDM2 binding protein antibody
    • MDM2 BP antibody
    • Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody
    • Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein antibody
    • Mdm2 transformed 3T3 cell double minute 2 p53 binding protein binding protein antibody
    • Mdm2-binding protein antibody
    • MDM2BP antibody
    • Mouse double minute 2 homolog binding protein antibody
    • MTBP antibody
    • MTBP_HUMAN antibody
    see all

Anti-MTBP antibody images

  • ab115529 at 10ug/ml staining MTBP in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115529 at 10ug/ml staining MTBP in Formalin-fixed, Paraffin-embedded Human tonsil tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-MTBP antibody (ab115529)

ab115529 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Sample Human Cell (cancer)
Specification cancer
Permeabilization Yes - 0.1% triton X 100 in pbs
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 28 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12%)
Sample Human Cell lysate - whole cell (cancer)
Specification cancer
Treatment 10uM piperlongumine 48hrs
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Miss. eunhye kim

Verified customer

Submitted Apr 09 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"