Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824)

Overview

  • Product name
    Anti-MTCO1 antibody [5D11-1C9] - N-terminal
  • Description
    Mouse monoclonal [5D11-1C9] to MTCO1 - N-terminal
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MTCO1 (N terminal).
    Database link: P00395

  • Positive control
    • WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn); Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 0.36% HEPES, 0.87% Sodium chloride
  • Concentration information loading...
  • Purification notes
    Purified from hybridoma cell culture supernatant by Protein L affinity chromatography from fetal bovine serum containing medium (Protein L does not bind bovine IgG).
  • Clonality
    Monoclonal
  • Clone number
    5D11-1C9
  • Isotype
    IgG2a
  • Light chain type
    kappa

Applications

Our Abpromise guarantee covers the use of ab219824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 57 kDa).

Images

  • All lanes : Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824) at 0.1 µg/ml

    Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
    Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-labeled Goat-anti-mouse IgG
    Developed using the ECL technique

    Predicted band size : 57 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

  • All lanes : Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824) at 2 µg/ml

    Lane 1 : Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn)
    Lane 2 : Whole cell extract HDFn-Rho0 cells depleted of mtDNA by long-term culture in the presence of ethidium bromide

    Lysates/proteins at 15 µg per lane.

    Secondary
    HRP-labeled Goat-anti-mouse IgG
    Developed using the ECL technique

    Predicted band size : 57 kDa

    Proteins were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

References

ab219824 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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