Our Abpromise guarantee covers the use of ab90668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa.


  • Function
    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
  • Pathway
    Energy metabolism; oxidative phosphorylation.
  • Involvement in disease
    Defects in MT-CO1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-CO1 are a cause of anemia sideroblastic acquired idiopathic (AISA) [MIM:516030]; a disease characterized by inadequate formation of heme and excessive accumulation of iron in mitochondria.
    Defects in MT-CO1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in MT-CO1 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.
    Defects in MT-CO1 are a cause of deafness sensorineural mitochondrial (DFNM) [MIM:500008]. DFNM is a form of non-syndromic deafness with maternal inheritance. Affected individuals manifest progressive, postlingual, sensorineural hearing loss involving high frequencies.
    Defects in MT-CO1 are a cause of colorectal cancer (CRC) [MIM:114500].
  • Sequence similarities
    Belongs to the heme-copper respiratory oxidase family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COI antibody
    • COX I antibody
    • COX1 antibody
    • COX1_HUMAN antibody
    • COXI antibody
    • Cytochrome c oxidase polypeptide I antibody
    • Cytochrome c oxidase subunit 1 antibody
    • Cytochrome C Oxidase subunit I antibody
    • Mitochondrially encoded cytochrome c oxidase I antibody
    • MT CO1 antibody
    • MT-CO1 antibody
    • MTCO 1 antibody
    • MTCO1 antibody
    see all


  • All lanes : Anti-MTCO1 antibody (ab90668) at 1 µg/ml

    Lane 1 : Whole cell lysates prepared from MCF-7 cells.
    Lane 2 : Whole cell lysates prepared from Hela cells.
    Lane 3 : Whole cell lysates prepared from Jurkat cells.
    Lane 4 : Whole cell lysates prepared from SMMC cells.
    Lane 5 : Whole cell lysates prepared from Colo320 cells.

    Lysates/proteins at 50 µg per lane.

    All lanes : HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size: 57 kDa


This product has been referenced in:
  • Celardo I  et al. dATF4 regulation of mitochondrial folate-mediated one-carbon metabolism is neuroprotective. Cell Death Differ 24:638-648 (2017). Read more (PubMed: 28211874) »
  • Zhang X  et al. Induction of mitochondrial dysfunction as a strategy for targeting tumour cells in metabolically compromised microenvironments. Nat Commun 5:3295 (2014). WB . Read more (PubMed: 24548894) »

See all 3 Publications for this product

Customer reviews and Q&As

I am sorry for the delay in getting back to you.

I have now checked the other antibodies we have against this target and unfortunately none would be predicted to cross react with the Drosophila protein except the rabbit polyclonal ab90668. Ho...

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Thank you for contacting us and your interest in our products.

We have not tested the Anti-MTCO1 antibody [1D6E1A8] (ab14705) for its cross reactivity with Drosophila melanogaster. The antibody has been raised against the full length human MT...

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Western blot
Human Cell lysate - other (Mitochondrial fractions of HeLa WT and HeLa rho-0)
Loading amount
26 µg
Mitochondrial fractions of HeLa WT and HeLa rho-0
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 17 2012

Vielen Dank für Ihre Antwort.

Es freut mich dass ich Ihnen weiterhelfen konnte.

Meine Vorhersage ist, dass Ihr Blot gutklappen wird, wenn das Kochen weggelassen wird.

Ich werde Ihr Feedback bezüglich der Datenbl&...

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Vielen Dank für Ihre Anfrage und die Zusendung Ihres Blots.

Ich freue mich Ihnen bestätigen zu können, dass MTCO1 immer unter der errechneten Größe von 57kDa läuft.

MTCO1 ist ein extrem hydrophobes Pro...

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Thank you for contacting us. These antibodies are indeed raised in mouse and these antibodies will work better in human, mouse, rat cells and tissue sections. In case you are planning to use these antibodies on mouse tissue sections then we wou...

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