Overview

  • Product nameAnti-MTCO2 antibody [12C4F12]
    See all MTCO2 primary antibodies
  • Description
    Mouse monoclonal [12C4F12] to MTCO2
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Rat, Zebrafish
  • Immunogen

    Full length native protein (purified) corresponding to Human MTCO2. Full length native Human Cytochrome C oxidase subunit II purified by SDS PAGE.

  • Positive control
    • WB: Isolated mitochondria from human heart. ICC/IF: Cultured human embryonic lung derived fibroblasts. Flow Cyt: HeLa cells.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    Alternative versions available:
    Anti-MTCO2 antibody (Alexa Fluor® 488) [12C4F12] (ab197958)
    Anti-MTCO2 antibody (HRP) [12C4F12] (ab197959)

Properties

Applications

Our Abpromise guarantee covers the use of ab110258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
ICC/IF Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal)
Flow Cyt Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
  • Involvement in diseaseDefects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
  • Sequence similaritiesBelongs to the cytochrome c oxidase subunit 2 family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COII antibody
    • COX2 antibody
    • COX2_HUMAN antibody
    • COXII antibody
    • Cytochrome c oxidase II antibody
    • Cytochrome c oxidase polypeptide II antibody
    • Cytochrome c oxidase subunit 2 antibody
    • MT CO2 antibody
    • MT-CO2 antibody
    • MTCO2 antibody
    see all

Anti-MTCO2 antibody [12C4F12] images

  • Anti-MTCO2 antibody [12C4F12] (ab110258) at 1 µg/ml + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg

    Predicted band size : 26 kDa
  • ab110258 staining human lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab110258 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 µg/ml. An Alexa Fluor® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.

  • ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue).

    An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).

References for Anti-MTCO2 antibody [12C4F12] (ab110258)

This product has been referenced in:
  • Cowan DB  et al. Intracoronary Delivery of Mitochondria to the Ischemic Heart for Cardioprotection. PLoS One 11:e0160889 (2016). IHC-P ; Rabbit . Read more (PubMed: 27500955) »
  • Zoladz JA  et al. Endurance training increases the efficiency of rat skeletal muscle mitochondria. Pflugers Arch 468:1709-24 (2016). WB . Read more (PubMed: 27568192) »

See all 76 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 4°C
Sample Zebrafish Cell (Whole Body)
Specification Whole Body
Permeabilization Yes - Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 25 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Rat Cell lysate - whole cell (Primary Neurons)
Specification Primary Neurons
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Aug 22 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Zebrafish Tissue lysate - whole (Larvae 5hpf)
Specification Larvae 5hpf
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Aug 22 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (15% GEL)
Sample Human Cell lysate - whole cell (H460)
Specification H460
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 26 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (15% GEL)
Sample Mouse Cell lysate - whole cell (mouse Embryonic Fibroblasts)
Specification mouse Embryonic Fibroblasts
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Cardiac Muscle)
Specification Cardiac Muscle
Fixative Paraformaldehyde
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Apr 08 2013

Thank you for contacting us.

The immunogens are as follows;

ab79393; A synthetic peptide corresponding to residues in human Cytochrome C oxidase subunit II
ab109739; A synthetic peptide corresponding to residues in Human Cytochro...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa WT vs HeLa rho-0)
Loading amount 13 µg
Specification HeLa WT vs HeLa rho-0
Gel Running Conditions Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted May 16 2012

Thank your for your inquiry.


Unfortunately, I am unable to answer your questions. This precise epitope of this antibody was not mapped by our lab. We currently also do not know of anyone that did map the binding site of this antibody. Read More

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"