Overview

  • Product nameAnti-MTCO2 antibody [EPR3314]
    See all MTCO2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3314] to MTCO2
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in human Cytochrome C oxidase subunit II

  • Positive control
    • K562, MCF7, THP1 and HeLa cell lysates; human heart, liver and heart muscle tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab79393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/5000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 25 kDa).
IP 1/30.
IHC-P 1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
  • Involvement in diseaseDefects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
  • Sequence similaritiesBelongs to the cytochrome c oxidase subunit 2 family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COII antibody
    • COX2 antibody
    • COX2_HUMAN antibody
    • COXII antibody
    • Cytochrome c oxidase II antibody
    • Cytochrome c oxidase polypeptide II antibody
    • Cytochrome c oxidase subunit 2 antibody
    • MT CO2 antibody
    • MT-CO2 antibody
    • MTCO2 antibody
    see all

Anti-MTCO2 antibody [EPR3314] images

  • All lanes : Anti-MTCO2 antibody [EPR3314] (ab79393) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : THP1 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 25 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
  • ab79393 at 1/100 dilution staining Cytochrome C oxidase subunit II in Human Liver (A), Human Heart(B) and HeLa Cell Line(C) by Immunohistochemistry, Paraffin-embedded tissues. Detection method of A and B was HRP-conjugated anti-rabbit with DAB substrate used for staining.

  • ICC/IF image of ab79393 stained HepG2 cells. The cells were 100% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79393, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HepG2 cells stained with ab79393 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79393, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-MTCO2 antibody [EPR3314] (ab79393)

This product has been referenced in:
  • Cowan DB  et al. Intracoronary Delivery of Mitochondria to the Ischemic Heart for Cardioprotection. PLoS One 11:e0160889 (2016). IHC-P ; Rabbit . Read more (PubMed: 27500955) »
  • Yan J  et al. Effects of mitochondrial translocation of telomerase on drug resistance in hepatocellular carcinoma cells. J Cancer 6:151-9 (2015). Read more (PubMed: 25561980) »

See all 9 Publications for this product

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The immunogens are as follows;

ab79393; A synthetic peptide corresponding to residues in human Cytochrome C oxidase subunit II
ab109739; A synthetic peptide corresponding to residues in Human Cytochro...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa WT vs HeLa rho-0)
Loading amount 13 µg
Specification HeLa WT vs HeLa rho-0
Gel Running Conditions Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Jul 31 2012

Thank you for contacting Abcam.


For IHC and ICC, antibodies are typically diluted in PBS or in a 1-3% BSA in PBS solution. Some researchers will use TBS instead, but either is fine. I have included links below to our IHC and ICC protoco...

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Thank you for your enquiry.

I have confirmed that ab79393, anti-Cytochrome C oxidase subunit II antibody antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue cultur...

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The immunogen of ab79393 shares 70% identity with rat Cytochrome c oxidase subunit 2. It also shares 70% identity with cat. Based on these number I would not predict cross-reactivity with either species.I have looked through the other antibodies in our...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (PBMC)
Loading amount 20 µg
Specification PBMC
Gel Running Conditions Reduced Denaturing
Blocking step Fluorescence special buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"