• Product nameAnti-MTCO3 antibody [DA5BC4]
    See all MTCO3 primary antibodies
  • Description
    Mouse monoclonal [DA5BC4] to MTCO3
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae
  • Immunogen

    Full length native Cytochrome C Oxidase subunit III (Saccharomyces cerevisiae).

  • Positive control
    • Isolated mitochondria from Human , Rat and Mouse hearts.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.



Our Abpromise guarantee covers the use of ab110259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 10-1 µg/mg of lysate.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 30 kDa.


  • FunctionSubunits I, II and III form the functional core of the enzyme complex.
  • Involvement in diseaseDefects in MT-CO3 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-CO3 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in MT-CO3 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.
  • Sequence similaritiesBelongs to the cytochrome c oxidase subunit 3 family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COIII antibody
    • COX3 antibody
    • COX3_HUMAN antibody
    • COXIII antibody
    • Cytochrome c oxidase polypeptide III antibody
    • Cytochrome c oxidase subunit 3 antibody
    • MT CO3 antibody
    • MT-CO3 antibody
    • MTCO3 antibody
    see all

Anti-MTCO3 antibody [DA5BC4] images

  • All lanes : Anti-MTCO3 antibody [DA5BC4] (ab110259) at 4 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria Rat heart at 10 µg
    Lane 3 : Isolated mitochondria from Mouse heart at 10 µg

    Predicted band size : 30 kDa

References for Anti-MTCO3 antibody [DA5BC4] (ab110259)

This product has been referenced in:
  • Zhao C  et al. Geniposide Protects Primary Cortical Neurons against Oligomeric Aß1-42-Induced Neurotoxicity through a Mitochondrial Pathway. PLoS One 11:e0152551 (2016). WB ; Mouse . Read more (PubMed: 27046221) »
  • Huang R  et al. Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV. PLoS One 9:e96246 (2014). Read more (PubMed: 24817082) »

See all 22 Publications for this product

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Application Western blot
Sample Human Cell lysate - other (HeLa WT vs HeLa rho-0)
Loading amount 13 µg
Specification HeLa WT vs HeLa rho-0
Gel Running Conditions Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (Mouse Liver Mitochondria)
Loading amount 20 µg
Specification Mouse Liver Mitochondria
Gel Running Conditions Reduced Denaturing (10% Bis Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 01 2011

Thank you for contacting Abcam. I will be glad to help you with your inquiry for a positive control for your antibodies. We do have several mitochondrial control lysates, which I have listed below: Mouse mitochondrial liver lysate...

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