Recombinant
RabMAb

Anti-MTERF antibody [EPR14202] (ab181836)

Overview

  • Product name
    Anti-MTERF antibody [EPR14202]
    See all MTERF primary antibodies
  • Description
    Rabbit monoclonal [EPR14202] to MTERF
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MTERF aa 250-350. The exact sequence is proprietary.
    Database link: Q99551

  • Positive control
    • HeLa, HepG2 and Jurkat cell lysate. Human liver and mouse muscle tissue. Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR14202
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab181836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Probably requires one or more components for termination activity.
  • Sequence similarities
    Belongs to the mTERF family.
  • Domain
    Contains nine structural repeats of about 35 residues, where each repeat contains three helices. The repeats form a left-handed superhelical assembly with a solenoid structure that wraps itself around DNA.
  • Post-translational
    modifications
    Phosphoprotein with mostly four phosphate groups. While the DNA-binding activity is unaffected by the phosphorylation state, only the phosphorylated form of the protein is active for termination activity. Functioning seems to be regulated by phosphorylation.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • mitochondrial antibody
    • Mitochondrial transcription termination factor 1 antibody
    • mTERF antibody
    • MTERF_HUMAN antibody
    • Transcription termination factor antibody
    see all

Images

  • All lanes : Anti-MTERF antibody [EPR14202] (ab181836) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 46 kDa

    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Anti-MTERF antibody [EPR14202] (ab181836) at 1/10000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 46 kDa

    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling MTERF using ab181836 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Immunohistochemical analysis of paraffin embedded mouse muscle tissue sections labeling MTERF using ab181836 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Flow Cytometry analysis of Jurkat cells labeling MTERF using ab181836 at a 1/50 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

References

ab181836 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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