Overview

  • Product nameAnti-MTF1 antibody
    See all MTF1 primary antibodies
  • Description
    Mouse monoclonal to MTF1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: GTVYDRTTVL IEQDPGTLED EDDDGQCGEH LPFLVGGEEG FHLIDHEAMS QGYVQHIISP DQIHLTINPG STPMPRNIEG ATLTLQSECP ETKRKEVKRY , corresponding to amino acids 41-141 of Human MTF1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.

Please see specific references PMID: 22021037 and 18990686 regarding use of this antibody to detect endogenous human MTF1.

Target

  • FunctionActivates the metallothionein I promoter. Binds to the metal responsive element (MRE).
  • Sequence similaritiesContains 6 C2H2-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Metal regulatory transcription factor 1 antibody
    • Metal response element binding transcription factor 1 antibody
    • Metal responsive transcription factor 1 antibody
    • MRE binding transcription factor antibody
    • MRE-binding transcription factor 1 antibody
    • MRE-binding transcription factor antibody
    • MTF 1 antibody
    • MTF1 antibody
    • MTF1_HUMAN antibody
    • Transcription factor MTF-1 antibody
    • Transcription factor MTF1 antibody
    • Zinc regulatory factor antibody
    • ZRF antibody
    see all

Anti-MTF1 antibody images

  • Western blot against tagged recombinant protein immunogen using ab55522 MTF1 antibody at 1ug/ml. Predicted band size of the recombinant immunogen is 37 kDa.
    We have only tested this antibody against the reecombinant protein.
    Please see specific references PMID: 22021037 and 18990686 regarding use of this antibody to detect endogenous human MTF1.

References for Anti-MTF1 antibody (ab55522)

This product has been referenced in:
  • Liu YC  et al. The Role of Small Ubiquitin-like Modifier-interacting Motif in the Assembly and Regulation of Metal-responsive Transcription Factor 1. J Biol Chem 286:42818-29 (2011). WB ; Human . Read more (PubMed: 22021037) »
  • Bellingham SA  et al. Regulation of prion gene expression by transcription factors SP1 and metal transcription factor-1. J Biol Chem 284:1291-301 (2009). WB ; Human . Read more (PubMed: 18990686) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human MDM)
Loading amount 100 µg
Specification human MDM
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jan 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"