Overview

  • Product name
  • Description
    Mouse monoclonal to MTF1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: GTVYDRTTVL IEQDPGTLED EDDDGQCGEH LPFLVGGEEG FHLIDHEAMS QGYVQHIISP DQIHLTINPG STPMPRNIEG ATLTLQSECP ETKRKEVKRY , corresponding to amino acids 41-141 of Human MTF1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.

Please see specific references PMID: 22021037 and 18990686 regarding use of this antibody to detect endogenous human MTF1.

Target

  • Function
    Activates the metallothionein I promoter. Binds to the metal responsive element (MRE).
  • Sequence similarities
    Contains 6 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Metal regulatory transcription factor 1 antibody
    • Metal response element binding transcription factor 1 antibody
    • Metal responsive transcription factor 1 antibody
    • MRE binding transcription factor antibody
    • MRE-binding transcription factor 1 antibody
    • MRE-binding transcription factor antibody
    • MTF 1 antibody
    • MTF1 antibody
    • MTF1_HUMAN antibody
    • Transcription factor MTF-1 antibody
    • Transcription factor MTF1 antibody
    • Zinc regulatory factor antibody
    • ZRF antibody
    see all

Images

  • Western blot against tagged recombinant protein immunogen using ab55522 MTF1 antibody at 1ug/ml. Predicted band size of the recombinant immunogen is 37 kDa.
    We have only tested this antibody against the reecombinant protein.
    Please see specific references PMID: 22021037 and 18990686 regarding use of this antibody to detect endogenous human MTF1.

References

This product has been referenced in:
  • Liu YC  et al. The Role of Small Ubiquitin-like Modifier-interacting Motif in the Assembly and Regulation of Metal-responsive Transcription Factor 1. J Biol Chem 286:42818-29 (2011). WB ; Human . Read more (PubMed: 22021037) »
  • Bellingham SA  et al. Regulation of prion gene expression by transcription factors SP1 and metal transcription factor-1. J Biol Chem 284:1291-301 (2009). WB ; Human . Read more (PubMed: 18990686) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human MDM)
Loading amount
100 µg
Specification
human MDM
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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