Overview

  • Product nameAnti-MTFMT antibody
  • Description
    Mouse polyclonal to MTFMT
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    MTFMT (NP_640335.1, 1 a.a. ~ 304 a.a) full-length human protein.

  • Positive control
    • MTFMT transfected 293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab70100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 44 kDa).

Target

  • FunctionFormylates methionyl-tRNA in mitochondria. A single tRNA(Met) gene gives rise to both an initiator and an elongator species via an unknown mechanism.
  • Sequence similaritiesBelongs to the fmt family.
  • DomainComposed of an N- and a C-terminal domain. The N-terminal domain carries the tetrahydrofolate (THF)-binding site and the C-terminal domain is presumably involved in positioning the Met-tRNA substrate for the formylation reaction.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • FMT antibody
    • FMT_HUMAN antibody
    • FMT1 antibody
    • Methionyl tRNA formyltransferase, mitochondrial antibody
    • Methionyl-tRNA formyltransferase antibody
    • mitochondrial antibody
    • mitochondrial methionyl tRNA formyltransferase antibody
    • MtFMT antibody
    see all

Anti-MTFMT antibody images

  • All lanes : Anti-MTFMT antibody (ab70100) at 1/500 dilution

    Lane 1 : MTFMT transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    MTFMT transfected lysate at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

References for Anti-MTFMT antibody (ab70100)

ab70100 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and your interest in our products.

ab70100: It has been tested in Western blotin MTFMT transfected 293T cell line as the WB image indicates. We did not get any signal on non-transfected 293T lysate. I can confirm th...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"