MTH1 overexpression 293T lysate (whole cell) (ab94036)

Overview

  • Product nameMTH1 overexpression 293T lysate (whole cell)
  • Description
    MTH1 overexpression lysate
  • General notesab94036 is a 293T cell transfected lysate in which Human MTH1 has been transiently over-expressed using a pCMV-MTH1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details on how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA. Tissue specificity: Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes. Similarity: Belongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain. Developmental stage: In peripheral blood lymphocytes, expressed at much higher levels in proliferating cells than in resting cells. PTM: The N-terminus is blocked.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

MTH1 overexpression 293T lysate (whole cell) images

  • ab94036 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-MTH1 antibody (ab55527) at 1/500 dilution

    Lane 1 : MTH1 overexpression 293T lysate (whole cell) (ab94036)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for MTH1 overexpression 293T lysate (whole cell) (ab94036)

ab94036 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"