General notesab94036 is a 293T cell transfected lysate in which Human MTH1 has been transiently over-expressed using a pCMV-MTH1 plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more details on how the transfected lysate was prepared view preparation notes
BackgroundFunction: Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.
Tissue specificity: Widely expressed with highest expression in thymus, testis, embryo and proliferating blood lymphocytes.
Similarity: Belongs to the Nudix hydrolase family.
Contains 1 nudix hydrolase domain.
Developmental stage: In peripheral blood lymphocytes, expressed at much higher levels in proliferating cells than in resting cells.
PTM: The N-terminus is blocked.