Overview

  • Product name
  • Description
    Goat polyclonal to MTHFD1L
  • Host species
    Goat
  • Specificity
    Based on homology with the immunogen sequence, we believe this antibody will also react with MTHFD1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    DTNDRFLRKITIGQ

    with a Cysteine residue linker, corresponding to internal sequence amino acids 578-591 of Human MTHFD1L protein (NP_056255.2).

  • Positive control
    • Human cerebelum lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab116615 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab116615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 106 kDa.

Target

  • Function
    May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.
  • Tissue specificity
    Detected in most tissues, highest expression found in placenta, thymus and brain. Low expression is found in liver and skeletal muscle. Up-regulated in colon adenocarcinoma.
  • Pathway
    One-carbon metabolism; tetrahydrofolate interconversion.
  • Sequence similarities
    In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
    In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.
  • Domain
    This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 10-formyl-THF synthetase antibody
    • C1TM_HUMAN antibody
    • Formyltetrahydrofolate synthetase antibody
    • formyltetrahydrofolate synthetase domain containing 1 antibody
    • FTHFSDC1 antibody
    • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like antibody
    • mitochondrial antibody
    • Monofunctional C1-tetrahydrofolate synthase antibody
    • MTC1THFS antibody
    • MTHFD1L antibody
    • RP1-292B18.2 antibody
    see all

Images

  • Anti-MTHFD1L antibody (ab116615) at 0.1 µg/ml + Human Cerebellum lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 106 kDa

References

ab116615 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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